畜牧兽医学报2026，Vol.57Issue(6)：3157-3169,13.DOI:10.11843/j.issn.0366-6964.2026.06.016
鸡前脂肪细胞分化过程中BMP2、PDK1和LPL基因3'-UTR的动态变化研究
Dynamic Changes in the 3'-UTR Regions of BMP2,PDK1,and LPL Genes during Chicken Preadipocyte Differentiation
摘要
Abstract
This study aimed to identify dynamic changes in the 3'-UTRs of adipogenesis genes during the differentiation of chicken preadipocytes(ICP1).In this study,the chicken immortalized preadipocyte cell line(ICP1)was used as the experimental material.Cells were divided into 6 time-point groups(0,24,48,72,96,and 120 h).Adipocyte differentiation was induced with 160 μmol·L-1 oleic acid.Lipid droplet deposition at 0 and 120 h was observed by Oil Red O staining and quantified to evaluate adipogenesis.The mRNA expression levels of adipogenic marker genes(PPARγ and FABP4)and target genes(BMP2,PDK1,LPL,and LDAH)were detected by q RT-PCR.In addition,3'-RACE and sequencing were performed to verify the 3'-UTR length variants of the BMP2 and PDK1 genes.All experiments were conducted with 3 biological replicates and corresponding technical replicates.The results showed that the expression levels of these genes varied at different stages of differentiation.Among them,the expression levels of BMP2 and PDK1 were significantly higher in the early stage of differentiation(24-48 h)than in the later stage,whereas the expression levels of LPL and LDAH gradually increased with cell differentiation,reached a peak at 48 h,and then gradually decreased with prolonged differentiation time.During the differentiation of ICP1 cells,BMP2,PDK1,and LPL generated 5,4,and 4 transcripts with different 3'-UTR lengths,respectively.In addition,quantitative analysis showed that BMP2 and PDK1 mainly expressed long 3'-UTR transcripts at the early stage of differentiation(0 and 24 h).As differentiation progressed,both long and short transcripts of BMP2 were present and expressed at similar levels at 96 and 120 h,whereas PDK1 exhibited similar expression levels of long and short transcripts at 48,72,96,and 120 h.Further analysis indicated that,compared with 0 h,BMP2 and PDK1 tended to express transcripts with longer 3'-UTRs at 48 h.During differentiation,adipogenesis genes with different 3'-UTR long and short transcripts were expressed in chicken preadipocytes,and there was a significant difference in the expression of different 3'-UTRs.The findings provide new insights and directions for exploring the molecular mechanisms by which APA regulates gene transcription and subsequently influences cell differentiation.关键词
鸡/前脂肪细胞/3'-UTR/可变多聚腺苷酸化Key words
chicken/preadipocytes/3'-UTR/alternative polyadenylation(APA)分类
农业科技引用本文复制引用
黄琴,赵素娟,杜志强..鸡前脂肪细胞分化过程中BMP2、PDK1和LPL基因3'-UTR的动态变化研究[J].畜牧兽医学报,2026,57(6):3157-3169,13.基金项目
湖北省重大攻关项目(2023BAA029) （2023BAA029）
