油气田环境保护2026，Vol.36Issue(3)：41-47,7.DOI:10.3969/j.issn.1005-3158.2026.03.007
外源营养调控对石油污染土壤微生物修复的强化机制研究
Study on Strengthening Mechanism of Exogenous Nutrient Regulation for Microbial Remediation of Petroleum-Contaminated Soil
摘要
Abstract
Imbalanced C/N/P ratio in petroleum-contaminated soil is a key factor restricting microbial remediation efficiency.Using the high-efficiency composite degrading bacterial agent M(Bacillus sp.J1-8 and Pseudomonas aeruginosa J6-7 blended at volume ratio of 1:3)developed in previous research as the test strain,this paper systematically investigates the influences of exogenous nutrient regulation on petroleum hydrocarbon degradation,and reveals the variation of soil nutrient content and response characteristics of dehydrogenase activity during remediation.The results show that among six nitrogen and phosphorus combinations,the mixture of NH4Cl and KH2PO4 achieves optimal performance with a 20-day degradation rate of 58.43％.After further optimization of molar C:N:P ratio(calculated by TOC),the maximum degradation rate of 63.32％is obtained at 100:12,rising by 19.19％compared with the blank group,and the dehydrogenase activity reaches 1.53 µg TF/(g·h).Variation of soil nutrients indicates nitrification occurs during remediation;available nitrogen is consumed faster than available phosphorus,leading to an upward N/P ratio over time.To tackle nutrient deficiency in later degradation stage,supplementary feeding interval is optimized,and nutrient addition every 12 days is proven optimal,raising the 30-day degradation rate to 72.12％with peak dehydrogenase activity of 1.74 µg TF/(g·h).This research provides systematic technical solutions and theoretical support for nutrient management in microbial remediation of petroleum-polluted soil.关键词
石油污染土壤/微生物修复/营养调控/氮磷比例/补加周期/脱氢酶活性Key words
petroleum-contaminated soil/microbial remediation/nutrient regulation/N/P ratio/nutrient supplement interval/dehydrogenase activity分类
资源环境引用本文复制引用
孟凡伟,陈立杰,袁博峰,周虔彧,崔潇雨,欧阳振宇,杜良贞..外源营养调控对石油污染土壤微生物修复的强化机制研究[J].油气田环境保护,2026,36(3):41-47,7.基金项目
中国石油工程建设有限公司华东环境岩土工程分公司科学研究与技术开发项目"石油污染场地微生物修复限制因子研究及关键修复技术开发". （）
