医学新知2026，Vol.36Issue(6)：631-639,9.DOI:10.12173/j.issn.1004-5511.202604054
树鼩主动脉血管内皮细胞系的建立及其在呼吸道合胞病毒感染研究中的应用
Establishment of an immortalized tree shrew aortic endothelial cell line and its application in the study of respiratory syncytial virus infection
摘要
Abstract
Objective To establish a stably passaged tree shrew aortic endothelial cell line(TSAEC),and to explore its application in respiratory syncytial virus(RSV)infection research.Methods Primary aortic endothelial cells derived from tree shrews were isolated and cultured using the tissue explant adherence method,and an immortalized TSAEC was established through lentivirus-mediated SV40T gene transduction.Immunofluorescence staining was performed to detect the expression of endothelial cell markers,including von Willebrand factor(vWF)and platelet endothelial cell adhesion molecule-1(CD31),to characterize the cellular phenotype.Cell proliferative activity and passage stability were assessed using the Cell Counting Kit-8(CCK-8)assay.Furthermore,tree shrew lung fibroblasts(TSLFC),tree shrew vascular smooth muscle cells(TSVSMC),human laryngeal epidermoid carcinoma cells(Hep-2),and African green monkey kidney cells(Vero)were used as control cells to compare RSV replication capacity among different cell types following infection.Viral susceptibility of TSAEC was evaluated by measuring viral load,viral titer,and observing cytopathic effects.Results The isolated TSAECs showed a typical flat spindle shape and highly expressed vascular endothelial marker proteins vWF and CD31.Cell proliferation activity was detected by CCK-8 assay.The results showed that there was no significant difference in proliferation capacity between untransfected primary cells and P10 generation cells.All generations of cells entered the logarithmic growth phase in the middle of culture and still maintained stable cell morphology and high cell viability till P60 generation TSAECs.Compared with TSLFC,TSVSMC,Hep-2 and Vero cells,RSV had stronger replication ability in TSAECs,and typical syncytial-like lesions were observed after infection.Conclusion This study successfully established the immortalized TSAEC,which was highly susceptible to RSV and could be used for the research on RSV infection mechanism and antiviral medicine.关键词
树鼩主动脉血管内皮细胞/呼吸道合胞病毒/病毒感染/细胞模型/细胞病变效应Key words
Tree shrew aortic endothelial cells/Respiratory syncytial virus/Viral infection/Cellular model/Cytopathic effect分类
医药卫生引用本文复制引用
李娜,王文广,范胜涛,曾庆巍,沈丽娟,陆彩霞,赵远..树鼩主动脉血管内皮细胞系的建立及其在呼吸道合胞病毒感染研究中的应用[J].医学新知,2026,36(6):631-639,9.基金项目
云南省卫生健康委员会医学学科带头人培养计划(D-2024006) （D-2024006）
国家重点研发计划"实验动物质量精准监管与安全性评价关键技术研究"重点专项(2024YFF0728801) （2024YFF0728801）
云南省科技厅科技计划项目(202505AK340009) （202505AK340009）
