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金莲花总酚酸抗痤疮致病菌的体外活性及机制

胡继春石召华张鹏王琪吴习孟燕李明家郑国华孙金龙胡俊杰

医药导报2026，Vol.45Issue(6)：989-1000,12.

医药导报2026，Vol.45Issue(6)：989-1000,12.DOI:10.3870/j.issn.1004-0781.2026.06.007

金莲花总酚酸抗痤疮致病菌的体外活性及机制

In vitro Antibacterial Activity and Mechanism of Total Phenolic Acids from Trollius chinensis Bunge Against Acne Pathogenic Bacteria

胡继春 ¹石召华 ²张鹏 ³王琪 ¹吴习 ¹孟燕 ¹李明家 ⁴郑国华 ¹孙金龙 ⁵胡俊杰¹

作者信息

1. 湖北中医药大学药学院,武汉 430065||湖北时珍实验室,武汉 430065
2. 湖北时珍实验室,武汉 430065||湖北中医药大学中药资源与中药复方教育部重点实验室,武汉 430065
3. 湖北省中医院,武汉 430061
4. 马应龙制药集团有限公司,武汉 430064
5. 武汉理工大学,武汉 430070
折叠

摘要

Abstract

Objective To investigate the antibacterial activity and mechanism of action of total phenolic acids from Trollius chinensis Bunge(PATC)against Cutibacterium acnes(C.acnes)and Staphylococcus aureus(S.aureus).Methods The antibacterial activity of PATC was evaluated using the agar well diffusion method,broth microdilution method,growth curve anal-ysis,and time-kill assay.Cytotoxicity to human keratinocytes(HaCaT)was assessed via the CCK-8 assay.The antibacterial mechanism was explored through biofilm inhibition assays,assessments of cell wall and membrane integrity,membrane potential measurements,determination of intracellular reactive oxygen species(ROS)levels,and scanning electron microscopy observa-tions,and employed UPLC-Q-TOF/ESI-MS for component identification.Results PATC exhibited dose-dependent inhibitory effects against both C.acnes and S.aureus,with inhibition zone diameters of 17.9 mm and 21.2 mm,respectively,and showed no significant toxicity to HaCaT cells.Mechanistic studies demonstrated that PATC effectively inhibited biofilm formation,disrup-ted cell wall and membrane integrity,induced membrane depolarization,and triggered ROS accumulation.Additionally,the UP-LC-Q-TOF/ESI-MS analysis led to the identification of 23 chemical components in PATC.Conclusion PATC exerts its antibac-terial effects through multiple mechanisms,including disruption of cellular structures,interference with membrane function,and induction of oxidative damage.

关键词

金莲花/总酚酸/痤疮致病菌/抗菌机制/痤疮

Key words

Trollius chinensis Bunge/Total phenolic acids/Acne pathogenic bacteria/Antibacterial mechanism/Acne

分类

医药卫生

引用本文复制引用

胡继春,石召华,张鹏,王琪,吴习,孟燕,李明家,郑国华,孙金龙,胡俊杰..金莲花总酚酸抗痤疮致病菌的体外活性及机制[J].医药导报,2026,45(6):989-1000,12.

基金项目

湖北省自然科学基金资助项目(2023AFD127) （2023AFD127）

湖北省教育厅计划项目(T2023014) （T2023014）

湖北省中央引导地方科技发展专项资金(2023EGA002). （2023EGA002）

医药导报

ISSN：1004-0781

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