湖北医药学院学报2026，Vol.45Issue(3)：276-282,封2,8.DOI:10.13819/j.issn.2096-708X.2026.03.003
黄连外泌体联合沙门氏菌外膜囊泡缓解结肠炎及其作用机制研究
Ameliorative Effects of Coptis chinensis Exosomes Combined with Salmonella Outer Membrane Vesicles on Colitis
摘要
Abstract
Objective To explore the ameliorative effect and mechanism of Coptis chinensis exosomes(CCEs)combined with Salmonella outer membrane vesicles(SOMVs)on dextran sulfate sodium(DSS)-induced colitis in mice.Methods CCEs and SOMVs were prepared and characterized.A mouse model of DSS-induced colitis was established,and the mice were treated with CCEs,SOMVs alone or their combination.The disease activity index(DAI)and pathological changes were e-valuated.Serum inflammatory factors,intestinal barrier proteins and oxidative stress indicators in colon tissues were detec-ted.Results Both CCEs and SOMVs exhibited typical vesicle structures.Compared with the model group and the monother-apy groups,the combined group achieved the optimal improvement on colitis symptoms and pathological lesions(P＜0.01).The levels of serum TNF-α,IL-6 and IL-1β were significantly reduced.The expressions of Claudin-1,Occludin and SOD in colon tissues were markedly upregulated,while the content of MDA was downregulated.Conclusion The combina-tion of CCEs and SOMVs can ameliorate colitis in mice,and the mechanism involves synergistic anti-inflammation,intesti-nal barrier repair and attenuation of oxidative stress.关键词
黄连外泌体/沙门氏菌外膜囊泡/结肠炎/肠道屏障/氧化应激Key words
Coptis chinensis exosomes/Salmonella outer membrane vesicles/Colitis/Intestinal barrier/Oxidative stress引用本文复制引用
秦郡,昝雨欣,查俊晔,张淑淇,胡愈瑶,杨翱畅,史客松,郝明月..黄连外泌体联合沙门氏菌外膜囊泡缓解结肠炎及其作用机制研究[J].湖北医药学院学报,2026,45(3):276-282,封2,8.基金项目
湖北省自然科学基金(2026AFB028) （2026AFB028）
湖北医药学院人才启动金项目(2025QDJZR12) （2025QDJZR12）
