湖北医药学院学报2026，Vol.45Issue(3)：283-289,301,8.DOI:10.13819/j.issn.2096-708X.2026.03.004
下丘脑腹侧乳头体前核DAT阳性神经元对睡眠-觉醒的调控作用
Role of DAT-positive Neurons in the Ventral Premammillary Nucleus in Sleep-Wake Regulation
摘要
Abstract
Objective To explore the role and mechanism of the ventral premammillary nucleus(PMv)in the regulation of sleep-wake rhythms.Methods In vivo multi-channel recording combined with synchronous EEG/EMG recording was per-formed to verify the correlation between the PMv and sleep-wake states.c-Fos immunofluorescence staining was used to an-alyze changes in the activity of PMv neurons induced by sleep deprivation and to clarify the involvement of the PMv in the transition from sleep to wakefulness.Brain stereotactic injection and immunohistochemistry were employed to further identify the neuronal subtypes in the PMv responsible for this state transition.Optogenetics and chemogenetics were adopted to ana-lyze the modulatory effect of the PMv on wakefulness.Results The activity of PMv neurons was closely associated with sleep-wake states.Among the 165 recorded neurons,32.73%were highly active during wakefulness.The collective firing activi-ty of neurons in the wake state was higher than that in rapid eye movement(REM)and non-rapid eye movement(NREM)sleep(P＜0.0001).The expression of c-Fos in PMv neurons was elevated after sleep deprivation compared with the control group and the sleep deprivation recovery group(P＜0.01),indicating its involvement in the transition from sleep to wake-fulness.After sleep deprivation,dopamine transporter(DAT)accounted for(72.3±1.2)%of c-Fos-positive neurons in the PMv,suggesting that DAT-positive neurons are the key neuronal subtype mediating wakefulness in this brain region.Optogenetic activation of PMvDAT neurons facilitated the transition from NREM sleep to wakefulness in mice(P＜0.0001),and chemogenetic activation of these neurons prolonged the duration of wakefulness(P＜0.01).Conclusion The PMv in in-volved in the regulation of sleep-wake states,and DAT-positive neurons in this region promote the transition from NREM sleep to wakefulness and extend the duration of wakefulness.The PMv is involved in the regulation of the sleep-wake states.DAT+neurons promote the transition from NREM sleep to wakefulness and increase wakefulness duration.关键词
下丘脑腹侧乳头体前核/多巴胺转运体/睡眠/觉醒Key words
Ventral premammillary nucleus/Dopamine transporter/Sleep/Wakefulness引用本文复制引用
柯奇周,赖鹏舒,孟文洁,于红..下丘脑腹侧乳头体前核DAT阳性神经元对睡眠-觉醒的调控作用[J].湖北医药学院学报,2026,45(3):283-289,301,8.基金项目
湖北省自然科学基金(2024AFB720) （2024AFB720）
湖北医药学院人才启动金(2022QDJZR010) （2022QDJZR010）
