中草药2026，Vol.57Issue(13)：4979-4986,8.DOI:10.7501/j.issn.0253-2670.2026.13.004
排骨灵化学成分研究
Chemical constituents of Fissistigma bracteolatum
摘要
Abstract
Objective To investigate the chemical constituents of Fissistigma bracteolatum.Methods The 95％ethanol extract of F.bracteolatum was successively separated by solvent partitioning,macroporous resin,silica gel,MCI,Sephadex LH-20,ODS column chromatography,and semi-preparative HPLC to obtain individual compounds.Their structures were elucidated using modern spectroscopic techniques such as NMR and MS.An in vitro inflammatory model was established using lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages to evaluate the anti-inflammatory activity of the isolated compounds.Results A total of 22 compounds were isolated from F.bracteolatum,comprising of 12 flavonoids,8 amides,1 lignan,and 1 lactone.These were determined as:5,7-dihydroxy-6,8-dimethoxyflavone(1),5-hydroxy-7,8-dimethoxyflavone(2),quercetin(3),rutin(4),5,7,8-trimethoxydihydroflavone(5),5,6,8-trimethoxy-7-hydroxydihydroflavone(6),6,7-dimethoxy-8-hydroxydihydroflavone(7),(2SS)-7-hydroxy-6,8-dimethoxydihydroflavone(8),dihydroquercetin(9),aromadendrin(10),4,2',6'-trihydro xy-3',4'-dimethoxy-dihydrochalcone(11),2',6'-dihydroxy-4'-methoxydihydrochalcone(12),N-trans-sinapoylmethoxytyramine(13),N-[2-(4-hydroxy-3-methoxyphenyl)ethyl]-3-phenyl-2-propenamide(14),(E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide(15),N-trans-feruloyltyramine(16),N-trans-p-coumaroyltyramine(17),N-ciss-feruloyltyramine(18),cis-N-feruloyl-3-O-methyldopamine(19),cannabisin D(20),3-caffeoyl-2-methyl-D-erythronic acid-1,4-lactone(21),and(-)-syringaresinol(22).Conclusion Compounds 1,7,9-11,13-15,17,19,and 21 were isolated from the genus Fissistigma for the first time.The anti-inflammatory activity results demonstrated that compounds 5,9,13,and 16 significantly inhibited the release of nitric oxide in LPS-induced RAW264.7 macrophages.关键词
番荔枝科/排骨灵/双氢槲皮素/香橙素/N-反式-阿魏酰基酪胺/抗炎活性Key words
Annonaceae/Fissistigma bracteolatum Chatterjee/dihydroquercetin/aromadendrin/N-trans-feruloyltyramine/anti-inflammatory activity分类
医药卫生引用本文复制引用
李艳洁,薛贵民,岳晓锋,郭鑫鑫,刘艺,冯好,邓世明,陈辉,冯卫生,薛金凤..排骨灵化学成分研究[J].中草药,2026,57(13):4979-4986,8.基金项目
河南省优秀青年基金资助项目(252300421137) （252300421137）
河南省科学技术研究发展计划联合基金项目(242301420109) （242301420109）
河南省高校科技创新团队支持计划(24IRTSTHN039) （24IRTSTHN039）
河南省科技攻关项目(242102310520) （242102310520）
河南省高校重点科研项目(25A360024) （25A360024）
大学生创新创业训练计划项目(202410471008) （202410471008）
