中草药2026，Vol.57Issue(13)：5017-5033,17.DOI:10.7501/j.issn.0253-2670.2026.13.007
《金匮要略》中酸枣仁汤现代煎煮条件的确立及其与酸枣汤的物质基础和抗失眠、抗焦虑药效比较
Establishment of modern decoction conditions for Suanzaoren Decoction from Jin Gui Yao Lue and its comparation with Suanzao Decoction in material basis,anti-insomnia and anti-anxiety effects
摘要
Abstract
Objective Compare the differences in material basis and anti-insomnia and anti-anxiety efficacy between Suanzaoren Decoction(SZRD,酸枣仁汤)and Suanzao Decoction(SZD,酸枣汤)in the Jin Gui Yao Lue.Methods This study quantifies 20 chemical ingredients as evaluation indicators to establish the boiling methods of SZRD and SZD,based on ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry(UPLC-Qtrap-MS)technology.Moreover,the ultra-high performance liquid chromatography-orbitrap tandem mass spectrometry(UPLC-Orbitrap-MS/MS)metabolite identification method specific to the Chinese herbal medicine was used to identify and compare the chemical profile of the two decoctions.Lastly,the p-chlorophenylalanine(PCPA)induced insomnia model mice were used to evaluate and compare the efficacy of the two decoctions in anti-insomnia and anti-anxiety.Results The method of boiling Suanzaoren in ceramic-coated iron pot for 25 min and adding other herbs then continuing to boil for 40 min can match well with the original records in Jin Gui Yao Lue.When Suanzaoren and Suanzao fruit were both used with the amount of 2 litters in Han Dynasty,the contents of jujuboside A,jujuboside B,spinosin,6'''-feruloylspinosin,magnoflorine,pachymic acid and ligustilide in SZRD were over 6-fold higher than those in SZD,whereas the contents of liquiritigenin,isoliquiritin,isoliquiritigenin and timosaponin AⅢ in SZD were over 2 times than those in SZRD.Among the top 100 compounds with the strongest mass spectrometric signals detected in the two decoctions,42 compounds exhibited higher contents in SZD and 21 compounds were enriched in SZRD.Among the differential compounds,three isoquinoline alkaloids[(-)-isocorypalmine,cheilanthifoline and asimilobine],one pyrrolidine alkaloid(stachydrine)and one steroidal alkaloid(zhebeinine)were further identified.The relative abundances of cheilanthifoline and asimilobine in SZD were 5.2 times and 4.8 times higher than those in SZRD,respectively;meanwhile,the relative abundances of(-)-isocorypalmine,stachydrine and zhebeinine in SZD only reached 0.3,0.4 and 0.4 times the corresponding values in SZRD.The anti-insomnia and anti-anxiety efficacies of the two decoctions in the PCPA induced insomnia mice are similar to each other,and no statistically significant intergroup difference in their pharmacological efficacy was observed..Conclusion Under the original decoction method recorded in Jin Gui Yao Lue,SZRD and SZD show significant differences in material basis,yet their similar anti-insomnia and anti-anxiety effects under specific condition may result from the combined actions of multiple active components.关键词
酸枣/酸枣仁/酸枣仁汤/液-质联用/失眠/资源开发利用/酸枣仁皂苷A/酸枣仁皂苷B/斯皮诺素/木兰花碱/茯苓酸/藁本内酯/(-)-异延胡索单酚碱/碎叶紫堇碱/巴婆碱/水苏碱/浙贝宁分类
医药卫生引用本文复制引用
王朝平,唐志书,董佳琪,沈雪儿,李莹莹,陈亚运,蔡兴航,李晓,樊修和,于金高..《金匮要略》中酸枣仁汤现代煎煮条件的确立及其与酸枣汤的物质基础和抗失眠、抗焦虑药效比较[J].中草药,2026,57(13):5017-5033,17.基金项目
国家重点研发计划项目(2025YFC3509100) （2025YFC3509100）
国家自然科学基金项目(U24A20787) （U24A20787）
陕西省科技厅重点研发计划项目(2024SF-YBXM-520) （2024SF-YBXM-520）
陕西省教育厅重点科学研究计划项目(23JY025) （23JY025）
陕西省中医药管理局科研项目(2021-GJ-JC001) （2021-GJ-JC001）
秦创原中医药产业创新聚集区项目(L2024-QCY-ZYYJJQ-X209) （L2024-QCY-ZYYJJQ-X209）
