中草药2026，Vol.57Issue(13)：5043-5053,11.DOI:10.7501/j.issn.0253-2670.2026.13.009
基于超分子传感阵列的胶类中药特征研究及应用
Characteristic analysis and application of gelatinous Chinese medicines based on supramolecular sensing array
摘要
Abstract
Objective To establish a method for differentiating gelatinous Chinese medicines(GCMs)based on supramolecular sensing technology.Methods An indicator displacement assay(IDA)strategy was employed where test sample solutions of GCMs served as analytes and macrocyclic molecule-dye host-guest pairs with specific fluorescence signals were used as sensing pairs to construct a sensor array.The fluorescence intensity changes resulting from the interaction between different GCMs and the sensing units were detected and combined with pattern recognition methods for differentiation.Results Through screening host-guest sensing units with selective fluorescence responses to GCMs from a supramolecular library consisting of cyclodextrins,cucurbiturils,calixarenes,and biphen[n]arenes hosts combined with dyes,a quaternary supramolecular sensor array was constructed including quaternary ammonium salt calix[5]arene(QAC5A)·eosin Y(EY)(0.4 μmol/L vs 0.5 μmol/L),quaternary ammonium modified azocalix[4]arene(QAAC4A)·EY(1 μmol/L vs 1 μmol/L),dodecyl quaternary ammonium salt azo calix[4]arene(QAAC4A12C)·EY(0.6 μmol/L vs 1 μmol/L),and terphen[3]arene(WTP3)·fluorescein(F1)(0.3 μmol/L vs 0.6 μmol/L).Principal component analysis(PCA)demonstrated that Ejiao(Colla Corii Asini),Xin'ejiao(Colla Corii Suilli),Biejiajiao(Colla Carapacis Trionychis),Lugujiao(Colla Ossis Cervi),Guijiajiao(Colla Carapacis et Plastri Testudinis)or Lujiajiao(Colla Cornus Cervi)could be significantly distinguished within a 95％confidence interval.Conclusion This study developed a quaternary supramolecular sensor array integrated with pattern recognition analysis which enables rapid discrimination of various GCMs.关键词
胶类中药/超分子传感/指示剂置换分析/传感阵列/区分/主成分分析/阿胶/新阿胶/鳖甲胶/鹿骨胶/龟甲胶/鹿角胶分类
医药卫生引用本文复制引用
郑建校,牛可敬,周立欣,王跃飞,于卉娟..基于超分子传感阵列的胶类中药特征研究及应用[J].中草药,2026,57(13):5043-5053,11.基金项目
国家中医药管理局科技项目(GZY-KJS-2025-062) （GZY-KJS-2025-062）
天津市科技计划项目(25ZYCGCG00390) （25ZYCGCG00390）
天津市科技计划项目(25HHZYSS00005) （25HHZYSS00005）
