中草药2026，Vol.57Issue(13)：5054-5068,15.DOI:10.7501/j.issn.0253-2670.2026.13.010
锰离子掺杂灵芝碳点的制备、表征与协同光热抑菌性能研究
Preparation,characterisation and synergistic photothermal antibacterial performance of manganese ion-doped ganoderma carbon dots
摘要
Abstract
Objective To screen for the most effective antimicrobial carbon dots from the traditional Chinese medicines Baiji(Bletilla striata),Cili(Rosa roxburghii),Lingzhi(Ganoderma lucidum)and Tianma(Gastrodia elata),and to systematically investigate the physicochemical properties,photothermal performance,antibacterial activity and mechanism of manganese-doped G.lucidum carbon dots(Mn-GLCDs).Methods Four kinds of herbal carbon dots derived from B.striata,R.roxburghii,G.lucidum and G.elata were prepared by a conventional hydrothermal method.The carbon dots with optimal antibacterial activity were screened out,and the morphology,structure and in vitro photothermal performance of the manganese-doped carbon dots were characterized.The antibacterial performance against Staphylococcus aureus and Escherichia coli under near-infrared(NIR)irradiation was evaluated through zone of inhibition assays,bacterial co-culture experiments,and biofilm inhibition and disruption tests.The antibacterial mechanism was preliminarily investigated by intracellular active oxygen fluorescence detection using a DCFH-DA probe.Biosafety was assessed by hemolysis assay and cytotoxicity test.Intracellular reactive oxygen species(ROS)fluorescence measurement using the 2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA)probe was adopted to preliminarily elucidate its antibacterial mechanism.Hemolysis assays and cytotoxicity evaluations were conducted to verify their biological safety.Results GLCDs possessed remarkably stronger antibacterial activity than carbon dots prepared from B.striata,R.roxburghii and G.elata carbon dots.Mn-GLCDs had a particle size of approximately 5 nm,and their surfaces were rich in hydrophilic functional groups including-OH,-NH2,C=O,C-N-C and C-O-C.X-ray photoelectron spectroscopy(XPS)and inductively coupled plasma mass spectrometry(ICP-MS)confirmed that the mass fraction of Mn2+reached 0.83％,and Mn2+was stably present in the carbon dot framework in a coordinated form.The red shift of the UV-Vis absorption peak and the Raman ID/IG＞1 indicated that Mn2+doping narrowed the bandgap and increased the density of structural defects.Photothermal performance evaluation showed that Mn-GLCDs could rapidly heat up under 808 nm laser irradiation(1.5 W/cm2),with a photothermal conversion efficiency of 35.03％.The maximum temperature fluctuation in six cycles of heating-cooling stability test was less than 6％,indicating good photothermal stability.Fluorescence performance test showed a fluorescence quantum yield of 20.51％and excitation-dependent fluorescence emission.In vitro antibacterial experiments demonstrated that,compared with undoped G.lucidum carbon dots,Mn-GLCDs exhibited the strongest antibacterial effect against S.aureus and E.coli under near-infrared irradiation,and could significantly inhibit and disrupt bacterial biofilms.Intracellular active oxygen detection showed that upon near-infrared irradiation,Mn-GLCDs elevated the active oxygen levels in S.aureus and E.coli to 6.75-fold and 5.44-fold of the negative control group,respectively.Biosafety evaluation showed that the hemolysis rate of Mn-GLCDs was below 5％within the antibacterial concentration range,and Mn-GLCDs exhibited no obvious toxicity to L929 and RAW264.7 cells.Conclusion This study constructs for the first time Mn-GLCDs,which significantly enhance the photothermal conversion capability and antibacterial activity.The mechanism is related to the induction of intracellular active oxygen.This work provides an experimental basis for the development of novel traditional Chinese medicine-based nanoscale antibacterial agents.关键词
中药/灵芝/碳量子点/锰离子掺杂/光热性能/细菌生物膜/白及/刺梨/天麻/抗菌活性/金黄色葡萄球菌/大肠杆菌/溶血/细胞毒性/生物安全性分类
医药卫生引用本文复制引用
余梦竹,罗坤顺,肖吕浪,朱月,任得强,周英,汪祖华..锰离子掺杂灵芝碳点的制备、表征与协同光热抑菌性能研究[J].中草药,2026,57(13):5054-5068,15.基金项目
中药制剂与大健康产品开发贵州省科技创新领军人才工作站(黔科合平台KXJZ[2025]042) （黔科合平台KXJZ[2025]042）
贵州省现代中药创制全省重点实验室(黔科合平台[2025]019) （黔科合平台[2025]019）
贵州中医药大学纳米药物技术研究中心(贵中医ZX合字[2024]070) （贵中医ZX合字[2024]070）
