中草药2026，Vol.57Issue(13)：5244-5256,13.DOI:10.7501/j.issn.0253-2670.2026.13.026
基于HS-GC-IMS法分析不同基原川贝母药材挥发性成分及其标志物
Analysis of volatile components and markers from different original species of Fritillariae Cirrhosae Bulbus based on HS-GC-IMS
摘要
Abstract
Objective To investigate the composition and differential characteristics of volatile organic compounds(VOCs)in Fritillariae Cirrhosae Bulbus(FCB)from six legally authorized origins,and provide a reference for origin identification and quality evaluation of FCB.Methods Headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)was employed to analyze the volatile components in dried bulbs of Fritillaria cirrhosa,F.unibracteata,F.przewalskii,F.delavayi,F.taipaiensis and F.unibracteata var.Wabuensis.Qualitative matching was performed using the built-in NIST and IMS database of GC-IMS Library Search.Principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and hierarchical cluster analysis(HCA)were applied to evaluate the differences in volatile components among samples from different origins.Results A total of 90 volatile organic compounds were preliminarily identified,mainly including aldehydes,alcohols,ketones,esters,carboxylic acids,pyrazines,and sulfur-containing compounds.Among them,aldehydes,alcohols,and ketones were the major categories.PCA and OPLS-DA results indicated that FCB samples from the six origins could be well distinguished.Based on variable importance in projection(VIP)values,17 potential differential markers were screened out,including ethyl acetate,2-propanone,a-phellandrene,3-hydroxy-2-butanone,methyl acetate,2-propanethiol,2-isobutyl-3-methoxypyrazine,propyl acetate,3-methylbutanal,1-butanol,2-methyl-2-propenal,hexanal,3-methyl-l-pentanol,2-methylpropanoic acid,3-butenenitrile,benzaldehyde,and 2-octanone.Cluster heatmap analysis further demonstrated that the above differential compounds could reflect the volatile metabolic characteristics of FCB from different origins.Conclusion HS-GC-IMS combined with chemometrics can effectively characterize the differences in volatile organic compounds among FCB from different origins,providing technical reference for origin identification,quality evaluation,and characteristic component screening of FCB.关键词
川贝母/基原鉴别/顶空-气相色谱-离子迁移谱/挥发性有机物/差异标志物Key words
Fritillariae Cirrhosae Bulbus/origin identification/headspace-gas chromatography-ion mobility spectrometry/volatile organic compounds/differential markers分类
医药卫生引用本文复制引用
赖楠楠,颜永刚,彭亮,胡本祥,杨冰月,徐胜,杨洁,李铂,张小飞,周静,陆博..基于HS-GC-IMS法分析不同基原川贝母药材挥发性成分及其标志物[J].中草药,2026,57(13):5244-5256,13.基金项目
国家重点研发计划资助(2023YFD1600402,2024YFC3506400) （2023YFD1600402,2024YFC3506400）
陕西省科技厅社发重点项目(No.2024SF-GJHX-05) （No.2024SF-GJHX-05）
中央引导地方科技发展专项项目(2025ZY-XCZXZS-22,2024ZY-CGZY-12) （2025ZY-XCZXZS-22,2024ZY-CGZY-12）
陕西省中医药管理项目(2021-QYZL-02,2025-CXTD-01) （2021-QYZL-02,2025-CXTD-01）
