中国病理生理杂志2026，Vol.42Issue(6)：1127-1141,15.DOI:10.3969/j.issn.1000-4718.2026.06.009
粪肠球菌通过下调MOB3B促进结直肠癌的化疗耐药性
Enterococcus faecalis promotes chemotherapy resistance by down-regulating MOB3B in colorectal cancer
摘要
Abstract
AIM:To investigate the contribution of Enterococcus faecalis(E.faecalis)to chemoresistance in colorectal cancer(CRC)and uncover the underlying mechanisms.METHODS:Bioinformatics analyses were performed to evaluate the expression of MOB3B,an Mps-one binder coactivator(MOB)protein family member,and its clinical impli-cations in CRC patients.E.faecalis was co-cultured with CRC cells to assess its effect on MOB3B expression.MOB3B was overexpressed or silenced in CRC cells to determine its effects on cell viability and chemosensitivity.The LRRC19-de-pendent mechanism was investigated through additional bioinformatics analyses.Immunohistochemical staining of clinical CRC tissues was performed to correlate MOB3B expression with Tumour Regression Grade.RESULTS:MOB3B down-regulation was associated with adverse clinicopathological characteristics and poor prognosis in CRC patients.Co-culture with E.faecalis down-regulated MOB3B expression in CRC cells.MOB3B overexpression decreased cell viability,while its silencing increased viability.MOB3B overexpression also reversed chemoresistance in CRC cells.Bioinformatics analy-ses revealed that MOB3B modulated resistance to oxaliplatin and 5-fluorouracil in an LRRC19-dependent manner.Low MOB3B expression correlated with high Tumour Regression Grade in clinical tissues.CONCLUSION:These findings in-dicate that Enterococcus faecalis promotes chemotherapy resistance by down-regulating MOB3B in colorectal cancer,and that MOB3B may serve as a potential marker for evaluating chemosensitivity and prognosis in CRC patients.The role of E.faecalis abundance as a clinical biomarker requires further validation in prospective cohorts.关键词
粪肠球菌/结直肠癌/化疗耐药性/MOB3B蛋白Key words
Enterococcus faecalis/colorectal cancer/chemoresistance/MOB3B protein分类
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张韦洋,华兴,周蕊,于莉娜,齐婧汝,赵卓扬,杨龙安,闫雍容,李雨璇,帅欣然,吴共发,宋嘉伟..粪肠球菌通过下调MOB3B促进结直肠癌的化疗耐药性[J].中国病理生理杂志,2026,42(6):1127-1141,15.基金项目
Supported by the National Natural Science Foundation of China(No.82073026),the Guangdong Natural Science Foundation(No.2024A1515012738),the Basic Research Project of Guangzhou High-Level University(No.2023A03J0606),and the Ganzhou"Science+Medicine"Joint Project(No.2025YLCE0005). （No.82073026）
