中国草地学报2026，Vol.48Issue(6)：1-20,20.DOI:10.16742/j.zgcdxb.20250420
370份冰草属种质资源表型多样性分析、综合评价及核心种质构建
Phenotypic Diversity Analysis,Comprehensive Evaluation,and Core Germplasm Construction of 370 Agropyron Accessions
摘要
Abstract
Using 370 Agropyron germplasms as experimental materials,this study systematically inves-tigated their genetic diversity using 31 phenotypic traits and multiple statistical methods,including coeffi-cient of variation,genetic diversity index,cluster analysis,correlation analysis,and grey relational analy-sis.Additionally,QGA software was employed to simulate and analyze genetic distance,sampling ratio,and clustering algorithms to determine optimal parameters for core germplasm construction.The effective-ness of the core subset was verified through principal component analysis and genetic diversity parameters.Results indicated significant phenotypic differentiation and rich genetic diversity in Agropyron germplasms.Quantitative traits(23 in total,with an average diversity index of 1.867)served as the primary carriers of genetic diversity,with their genetic diversity index was significantly higher than that of qualitative traits(1.328).Phenotypic correlations revealed a"source-sink-flow"coordinated development mechanism:leaves and stems exhibited coordinated growth;spike length was positively correlated with spikelet and flo-ret numbers but negatively correlated with spikelet density,providing a basis for ecological adaptive breed-ing.Cluster analysis categorized the materials into three characteristic germplasm types:Type Ⅰ(24 resources,characterized by tall plants,long roots,and long spikes),Type Ⅱ(123 resources,emphasiz-ing spike and leaf development),and Type Ⅲ(223 resources,with high spikelet density and numerous glume veins).Grey relational analysis identified seven core evaluation indicators:spikelet number,florets per spike,spike width,first rachis internode length,leaf color,leaf sheath length,and second internode stem diameter.Fifteen comprehensively excellent materials(GB3,BB34,MB15,BB55,XB6,MB25,MB32,GB11,MB7,SB55,SB52,BB68,XB3,BB110,XB2)were selected.A core germplasm consist-ing of 92 accessions was constructed using"Mahalanobis distance+deviation sampling+sum of squared deviations+25%sampling ratio."This core subset included all 15 excellent materials,achieving an organic unity of genetic diversity conservation(MD=0,CR=100%)and superior trait screening(VD=41.935%,VR=115.413%).Principal component analysis confirmed high consistency between the core germplasm and the original population in genetic structure(core germplasm cumulative contribution rate:73.014%;original population:64.407%).This study integrates phenotypic diversity analysis,grey relational evalua-tion,and core germplasm construction techniques,providing theoretical basis and material support for the efficient utilization and genetic improvement of Agropyron germplasm resources.关键词
冰草属/种质资源/表型多样性/灰色关联度/核心种质Key words
Agropyron/Germplasm resources/Phenotypic diversity/Grey relational degree/Core germplasm分类
农业科技引用本文复制引用
吴昌忠,潘文喧,赵彦,闫伟红,霍春燃,闫文昊,李志勇,武自念..370份冰草属种质资源表型多样性分析、综合评价及核心种质构建[J].中国草地学报,2026,48(6):1-20,20.基金项目
内蒙古自治区科技计划"锡林郭勒盟草地多功能兼用型乡土草高效繁育与加工利用技术集成"(2022YFDZ0083) （2022YFDZ0083）
内蒙古农业大学一流学科专项重大项目(YLXKZX-NND-030) （YLXKZX-NND-030）
内蒙古科技重大专项"优良乡土草种质创新与应用关键技术研究"(2021ZD0031) （2021ZD0031）
内蒙古自治区自然科学基金面上项目"扁穗冰草耐盐性相关分子标记开发及辅助新种质创制"(2023MS03033) （2023MS03033）
内蒙古自治区揭榜挂帅项目(2022JBGS0014) （2022JBGS0014）
