中国草地学报2026，Vol.48Issue(6)：21-32,12.DOI:10.16742/j.zgcdxb.20250438
红三叶TpLHC基因家族分析以及铅胁迫下的表达模式
Identification and Expression Mode of the TpLHC Gene Fam-ily in Red Clover under Lead Stress
摘要
Abstract
Lead contamination is a major abiotic stress that reduces crop yield,causes plant death,and degrades the environment.Red clover(Trifolium pratense)is an important forage legume with substantial potential for lead tolerance.Based on previously generated transcriptome data from red clover exposed to different lead concentrations,the present study identified and characterized the LHC gene family using bio-informatics approaches.A total of 21 TpLHC genes were identified and assigned to the LHCA(5 mem-bers)and LHCB(16 members)families.Phylogenetic analysis of TpLHC proteins and their homologs in Arabidopsis thaliana and Medicago truncatula classified the TpLHCA and TpLHCB families into five and seven subfamilies,respectively.Subcellular localization analysis predicted that most TpLHC proteins were localized to chloroplasts,whereas TpLHCA6 and TpLHCB7 were localized to the plasma membrane.Expression analysis under 0,500,1 000,2 000,and 3 000 mg/kg lead treatments showed that,after 45 d of exposure,expression of all TpLHCB8 subfamily members was strongly suppressed under 500 mg/kg lead stress,TpLHCA1,TpLHCA6,TpLHCB1.1,TpLHCB1.6,TpLHCB4.1,TpLHCB5,and TpL-HCB7 were upregulated under 3 000 mg/kg lead stress.qRT-PCR analysis showed that,after 48 h of exposure to 1 000 mg/kg lead,all LHCA family members except TpLHCA6 were downregulated.Among the LHCB genes,except for TpLHCB1.2 and TpLHCB7,the expression levels of the remaining members increased at 48 h under stress compared to those under non-stress conditions,suggesting that LHCB genes may be more strongly associated with lead-stress tolerance.关键词
红三叶/LHC基因家族/生物信息学分析/叶绿体/铅胁迫Key words
Red clover/LHC gene family/Bioinformatics/Chloroplast/Lead stress分类
农业科技引用本文复制引用
姜丁予,闫旭,龙羲,杨明浩,王召明,姜婧雯,邢智翔,官彩倩,殷秀杰..红三叶TpLHC基因家族分析以及铅胁迫下的表达模式[J].中国草地学报,2026,48(6):21-32,12.基金项目
草业国家技术创新中心重大创新平台建设专项(CCPTZX2024GJ01-1-2) （CCPTZX2024GJ01-1-2）
国家自然科学基金项目(32471760) （32471760）
国家重点研发计划(2023YFD1200302) （2023YFD1200302）
黑龙江省联合重点基金项目(ZL2024C018) （ZL2024C018）
龙江科技英才创新类人才(RC2025CX005) （RC2025CX005）
