中国农业科技导报2026，Vol.28Issue(6)：32-39,8.DOI:10.13304/j.nykjdb.2025.0455
基于CRISPR/Cas9基因编辑技术研究AT1同源基因对小麦耐盐碱性的影响
Effect of AT1 Homolog in Wheat to Saline-alkali Tolerance Based on CRISPR/Cas9
摘要
Abstract
alkaline tolerance 1(AT1),a gene cloned from sorghum,is involved in alkaline tolerance.In wheat,AT1 homologs are located on chromosomes 4A,7A and 7D.To explore the role of AT1 gene in improving salt-alkali tolerance of wheat,CRISPR/Cas9 gene editing technology was used to construct AT1 gene knockout lines with the wheat variety'Fielder'as the background.A total of 8 gene-edited lines were obtained,among which AT1 homologous genes on chromosomes 4A,7A and 7D were all edited in 6 lines.Plants were subjected to neutral salt stress(100 mmol·L-1),alkaline salt stress(60,125 mmol·L-1)and combined saline-alkali stress(135 mmol·L-1)and the seedling length,fresh weight and dry weight of wild-type plants and mutants were determined and analyzed.The results showed that there were differences in the significance levels of AT1 gene-edited materials under different stress treatments:the significance level was the lowest under salt stress,followed by that under saline-alkali stress,and the highest under alkali stress.Above results provided insights into the potential application of AT1 homologs in enhancing saline-alkali tolerance in wheat.关键词
小麦/耐盐碱/CRISPR/Cas9/alkaline tolerance 1Key words
wheat/salt-alkali resistance/CRISPR/Cas9/alkaline tolerance 1分类
农业科技引用本文复制引用
禹亚妮,赵齐,林冰冰,韩文政,刘琰,王岩,吴佳洁..基于CRISPR/Cas9基因编辑技术研究AT1同源基因对小麦耐盐碱性的影响[J].中国农业科技导报,2026,28(6):32-39,8.基金项目
国家重点研发计划项目(2022YFD1201700). （2022YFD1201700）
