中国农业科技导报2026，Vol.28Issue(6)：54-62,9.DOI:10.13304/j.nykjdb.2024.0787
巨紫荆叶绿体基因组密码子偏好性分析
Analysis of Codon Bias in Chloroplast Genome of Cercis gigantea
摘要
Abstract
Cercis gigantea is one of the common tree species in landscaping.In order to understand the codon bias pattern of chloroplast genome of Cercis gigantean and analyze the influencing factors,CodonW 1.4.2 and CUSP online software were used for neutral mapping analysis,ENC-plot analysis,PR2-plot analysis and cluster analysis of chloroplast genome of Cercis gigantean.The results showed that the average GC content of codons in chloroplast genome was 37.20%.The GC1 and GC2 were 46.34%and 39.28%,respectively,which was significantly higher than GC3(28.15%).This suggested that the codon of chloroplast genome of Cercis gigantea was more biased towards A and U on the final base.The mean value of the effective codon number was 47.06,indicating a weak codon bias.And the codon bias of chloroplast genome of Cercis gigantea was affected mainly natural selection,and also influenced by other factors.In addition,6 optimal codons were screened,which preferred to use bases of their high content.Above results provided basic data for the future genetic engineering,codon optimization and improvement of exogenous gene expression level of Cercis gigantea.关键词
巨紫荆/叶绿体基因组/密码子偏好性/最优密码子Key words
Cercis gigantea/chloroplast genome/codon bias/optimal codon分类
生物科学引用本文复制引用
马一丹,杨娅茹,郭超超,王婉茹,刘艺平,孔德政..巨紫荆叶绿体基因组密码子偏好性分析[J].中国农业科技导报,2026,28(6):54-62,9.基金项目
国家自然科学基金项目(31600568) （31600568）
河南省科技攻关项目(212102110185). （212102110185）
