中国蔬菜Issue(6)：38-50,13.DOI:10.19928/j.cnki.1000-6346.2026.0013
结球甘蓝游离小孢子培养技术研究进展
Research Progress on Isolated Microspores Cultivation Technology of Brassica oleracea L.
摘要
Abstract
Isolated microspore culture serves as a highly efficient breeding tool capable of rapidly generating homozygous doubled haploid(DH)materials,significantly shortening the breeding cycle.This technology holds substantial importance for improving the breeding efficiency of Brassica vegetables.This paper reviews the research progress of isolated microspore culture technology in China,specifically focusing on its applications in disease-resistant breeding,molecular marker-assisted breeding,and genetic resource innovation in Brassica oleracea L.It elaborates on the effects of key factors including donor genotype,selection of donor material at the optimal stage,heat shock treatment,medium optimization,plant growth regulators,and additives on embryoid induction and plant regeneration.Furthermore,the study proposes future prospects for further enhancing the efficiency and precision of Brassica oleracea L.through the optimization of the microspore culture technical system.关键词
结球甘蓝/游离小孢子培养/胚状体诱导/双单倍体/抗病育种/分子标记辅助育种/综述Key words
Brassica oleracea L./isolated microspore culture/embryoid induction/doubled haploid/disease resistance breeding/molecular marker-assisted breeding/review引用本文复制引用
罗鑫茂,赵文,宋江萍,蒲俊宏,杨晴,黎莉,冯雪,徐志勇,牟文平..结球甘蓝游离小孢子培养技术研究进展[J].中国蔬菜,2026,(6):38-50,13.基金项目
国家现代农业产业技术体系四川特色蔬菜创新团队建设专项资金资助项目(SCCXTD-2024-04) （SCCXTD-2024-04）
