中国蔬菜Issue(6)：106-120,15.DOI:10.19928/j.cnki.1000-6346.2026.3020
二倍体马铃薯种质资源筛选与评价
Screening and Evaluation of Diploid Potato Germplasm Resources
摘要
Abstract
Diploid potatoes are important germplasm resources.Screening and evaluating diploid potato germplasm resources can provide valuable materials for the development of superior new potato germplasm.To identify for self-compatible and high-yielding diploid potatoes,this study evaluated 68 potato germplasm resources(including 57 diploids and 11 tetraploids)for chromosome ploidy,self-compatibility,and field agronomic traits.Regarding ploidy identification,by comparing three methods-leaf length-width ratio,guard cell chloroplast counting,and flow cytometry-chloroplast counting was identified as a simple and effective method for determining potato ploidy.For self-compatibility identification,6 self-compatible accessions were identified using the Sli gene molecular marker,including 1 tetraploid accession and 5 diploid accessions.Through field self-pollination experiments,33 diploid self-compatible accessions were identified,4 of which contained the Sli gene molecular markers.The field agronomic evaluation results showed that the diploid accession G18-27-27 exhibited the highest average single-plant yield of 1.23 kg,and was self-compatible;R8209 produced the highest number of tubers per plant with 25.25 tubers;among the accessions carrying Sli gene marker,YN20S225-1 had the highest single-plant yield of 0.86 kg,and YN20S145-1 produced the highest number of tubers per plant,with 24.92 tubers.The evaluation methods screened and the potato germplasm resources evaluated in this study provide a technical reference and material basis for the improvement of diploid potato germplasm.关键词
二倍体马铃薯/倍性鉴定/自交亲和性鉴定/田间性状评价Key words
diploid potato/ploidy identification/self-compatibility identification/field trait evaluation引用本文复制引用
田媛,杨妍,卢丽丽,崔毕华,向成轶,段龙凤,李先平,蒋伟..二倍体马铃薯种质资源筛选与评价[J].中国蔬菜,2026,(6):106-120,15.基金项目
云南省科技计划项目(202304BI090026),云南省基础研究专项面上项目(202101AT070257),云南省农业基础研究联合专项面上项目(202301BD070001-124),云南省"兴滇英才支持计划"项目(XDYC-QNRC-2023-0455) （202304BI090026）
