中国饲料Issue(11)：106-112,7.DOI:10.15906/j.cnki.cn11-2975/s.2025010018-06
固相萃取-气相色谱-串联质谱法测定豆粕饲料中35种农药残留
Determination of 35 pesticide residues in soybean meal feed by solid phase extraction combined with gas chromatography-tandem mass spectrometry
摘要
Abstract
This study aimed to established a high-throughput analytical method combining solid-phase extraction pretreatment with gas chromatography-triple quadrupole tandem mass spectrometry(GC-MS/MS)for detecting 35 pesticide residues in soybean meal feed matrices.The target compounds were extracted using acetonitrile solvent and detected through GC-MS/MS in multiple reaction monitoring(MRM)mode,with quantification performed via external standard method.A graphitized carbon black/aminopropyl(Carb-GCB/NH2)composite cartridge purification system was employed to eliminate matrix interferences.Results demonstrated excellent linear relationships for all pesticides within the range of 0.01～0.50 mg/L,with correlation coefficients≥0.99.The method exhibited high sensitivity,showing a detection limit of 0.003 mg/kg and quantification limit of 0.01 mg/kg.At three spiked concentration levels(0.01,0.05,0.10 mg/kg),average recoveries ranged from 70.20%to 120.67%,with relative standard deviations(RSD)between 0.63%and 12.53%.This optimized approach simplifies operational procedures while maintaining good accuracy and reproducibility,proving suitable for rapid screening and quantitative analysis of multiple pesticide residues in feed products.关键词
气相色谱-质谱/农药残留/固相萃取/前处理/豆粕饲料Key words
GC-MS/MS/pesticides residual/solid-phase extraction/pretreatment/soybean meal feed分类
农业科技引用本文复制引用
刘慧玲,张冀平,康占省,王敬..固相萃取-气相色谱-串联质谱法测定豆粕饲料中35种农药残留[J].中国饲料,2026,(11):106-112,7.基金项目
海关总署科研项目(2023HK081) （2023HK081）
