中国饲料Issue(11)：123-131,177,10.DOI:10.15906/j.cnki.cn11-2975/s.2025010017-05
一株豆粕发酵高产蛋白酶芽孢杆菌的筛选及特性研究
Screening and characterization of a bacillus strain with high yield protease for soybean meal fermentation
摘要
Abstract
Soybean peptide made from soybean meal is a new protein modification product with better quality,which is widely used in pharmaceutical,food,cosmetics,feed preparation,and plant cultivation.At present,the production of soybean peptides by microbial fermentation is regarded as the most advanced method.In this study,a Bacillus strain with high protease production was selected from the intestinal contents of sea cucumber,and the conditions of protease production and liquid fermentation of soybean meal were optimized.The results showed that the strain was Bacillus velezensis(Bacillus DLH-1).When the medium composition was 2%beef paste,0.75%yeast powder and 1%NaCl,the culture temperature was 35℃,the initial pH 7.0,the inoculation amount was 5%and the culture time was 33 h,the proteinase-producing activity was 131.309 U/mL,which was 1.94 times higher than that before optimization.It was found that the soybean meal could be effectively hydrolyzed under the conditions of 4%inoculation amount,1:10 ratio of material to water,36 h fermentation hydrolysis time,45℃fermentation hydrolysis temperature and initial pH 6.0,and the polypeptide content was 1.76 times higher than that before optimization.The aim of this study was to provide high performance bacteria source for soybean meal fermentation to produce protein feed or fertilizer,and provide help for the development and utilization of soybean meal and the improvement of economic benefits of related enterprises.关键词
豆粕/蛋白酶/液态发酵/贝莱斯芽孢杆菌/条件优化Key words
soybean meal/protease/liquid fermentation/Bacillus velezensis/condition optimization分类
农业科技引用本文复制引用
杨攀凤,谢紫贤,王宝,赵治伟,王红英,钱斯日古楞..一株豆粕发酵高产蛋白酶芽孢杆菌的筛选及特性研究[J].中国饲料,2026,(11):123-131,177,10.基金项目
内蒙古自治区科技重大专项项目(222002023059) （222002023059）
