中国比较医学杂志2026，Vol.36Issue(11)：11-20,10.DOI:10.3969/j.issn.1671-7856.2026.11.002
长蛇灸通过激活PINK1/Parkin通路增强线粒体自噬改善"阳虚质"大鼠棕色脂肪组织产热功能
Long Snake Moxibustion improves thermogenic function of brown adipose tissue in rats with yang deficiency constitution by activating the PINK1/Parkin pathway to enhance mitophagy
摘要
Abstract
Objective To observe the effects of Long Snake Moxibustion on brown adipose tissue(BAT)thermogenesis in rats with a yang deficiency constitution,and explore the underlying mechanism involved.Methods Fifteen SPF-grade SD rats were randomly divided into normal,model,and Long Snake Moxibustion groups(five per group).The model and Long Snake Moxibustion groups underwent heat-preference screening and generational breeding to establish a yang deficiency constitution model.Following modeling,the Long Snake Moxibustion group received treatment for 15 consecutive days.Measurements included anal temperature,body weight,heat preference,ice-water swimming time,and spontaneous activity count.Serum levels of adrenocorticotropic hormone(ACTH),cortisol,and corticotropin-releasing hormone(CRH)were detected by enzyme-linked immunosorbent assay.Hematoxylin and eosin staining was used to observe lipid droplet morphology in interscapular BAT.The expression of uncoupling protein 1(UCP1),PTEN-induced putative kinase 1(PINK1),and Parkinson disease protein(Parkin)in BAT was detected by immunohistochemistry and Western blot analysis.Results Compared with the normal group,the model group exhibited decreased anal temperature,body weight,ice-water swimming time,and spontaneous activity(P＜0.01,P＜0.001),increased heat preference(P＜0.01),lower ACTH and CRH(P＜0.01),decreased BAT lipid droplet number with enlarged vacuoles,and downregulated protein expression of BAT,UCP1,PINK1,and Parkin(P＜0.001,P＜0.000 1).Compared with the model group,the Long Snake Moxibustion group showed increased anal temperature,body weight,ice-water swimming time,and spontaneous activity after treatment(P＜0.05,P＜0.01),decreased heat preference(P＜0.05),elevated ACTH and CRH(P＜0.01),increased BAT lipid droplet number with smaller vacuoles,and upregulated protein expression of BAT UCP1,PINK1,and Parkin(P＜0.05,P＜0.01,P＜0.001,P＜0.000 1).Conclusions Long Snake Moxibustion improved symptoms and serum hormone levels in rats with a yang deficiency constitution.The mechanism may be related to PINK1/Parkin signaling activation to promote mitophagy,increasing BAT UCP1 expression and enhancing thermogenesis.关键词
长蛇灸/阳虚质/大鼠/棕色脂肪组织/线粒体自噬Key words
Long Snake Moxibustion/yang deficiency constitution/rats/brown adipose tissue/mitophagy分类
医药卫生引用本文复制引用
黄辉,胡秀武,李字卿,金梦雨,黄璟,耿乐乐,唐丽梅,余希婧,刘航,黄平..长蛇灸通过激活PINK1/Parkin通路增强线粒体自噬改善"阳虚质"大鼠棕色脂肪组织产热功能[J].中国比较医学杂志,2026,36(11):11-20,10.基金项目
国家自然科学基金(82560893) （82560893）
江西省中医药管理局科技计划(2020B0346) （2020B0346）
南昌市科技局医疗卫生科技支撑项目(2022-KJZC-019) （2022-KJZC-019）
江西省自然科学基金青年基金(20242BAB20449). （20242BAB20449）
