中国比较医学杂志2026，Vol.36Issue(11)：46-54,9.DOI:10.3969/j.issn.1671-7856.2026.11.005
环状八肽OCP2对高原缺氧小鼠心肌组织损伤的保护作用研究
Effect of cyclic octapeptide OCP2 on myocardial tissue damage in mice exposed to high-altitude hypoxia
摘要
Abstract
Objective Exploring the protective effects and potential molecular mechanisms of cyclic peptide OCP2 on myocardial injury in mice exposed to high altitude hypoxia.Methods Sixty male BALB/c mice were divided randomly into Control,Model,acetazolamide(ACZ),and OCP2 low-,medium-,and high-dose(OCP2-L/M/H)groups.After single intraperitoneal administration of the corresponding medication for 10 minutes,each group was placed in a simulated altitude environment of 8 000 m.Heart tissue was collected 24 hours later to observe pathological changes in myocardial tissue and measure levels of oxidative stress and inflammatory factors,including hypoxia-inducible factor 1α(HIF-1α),nuclear factor erythroid 2-related factor 2(Nrf2),peroxisome proliferator-activated receptor γ coactivator 1-α(PGC-1α),and peroxisome proliferator-activated receptor γ(PPARγ).Results Compared with the Control group,myocardial tissue cells in the Model group were disordered and swollen,with lighter staining and significantly higher pathological scores(P＜0.01).Superoxide dismutase(SOD),catalase(CAT),and glutathione(GSH)levels were significantly decreased(P＜0.01),malondialdehyde(MDA),tumor necrosis factor(TNF)-α,interleukin(IL)-1 β,IL-6,HIF-1α,and Nrf2 expression levels were significantly increased(P＜0.01),and PGC-1α and PPARγ levels were markedly decreased(P＜0.05 or P＜0.01).Compared with the Model group,pathological scores of cardiac tissue in each OCP2-dose group were significantly reduced(P＜0.05 or P＜0.01)and muscle fiber swelling and misalignment were alleviated.The SOD,CAT,and GSH contents and expression levels of PGC-1α and PPARγ were significantly increased(P＜0.05 or P＜0.01),while the TNF-α,IL-1β,IL-6,and MDA contents HIF-1α,and Nrf2 expression levels were significantly decreased(P＜0.05 or P＜0.01).Conclusions OCP2 can protect against myocardial tissue damage in high-altitude hypoxic mice,possibly via the effective inhibition of HIF-1α activity,thereby disrupting its synergistic effect with Nrf2 and PPARγ/PGC-1αsignaling axes,ultimately inhibiting the production of inflammatory factors and improving antioxidant capacity.关键词
环状八肽OCP2/高原缺氧/小鼠/心肌组织Key words
cyclic octapeptide OCP2/high-altitude hypoxia/mouse/myocardial tissue分类
医药卫生引用本文复制引用
张佩,魏旭,章自会,蒲中枢,张梅,高玉海,申栋帅,陈克明..环状八肽OCP2对高原缺氧小鼠心肌组织损伤的保护作用研究[J].中国比较医学杂志,2026,36(11):46-54,9.基金项目
甘肃省科技重大专项(24ZDFA008) （24ZDFA008）
甘肃省自然科学基金(24JRRA010,25JRRA432). （24JRRA010,25JRRA432）
