中国比较医学杂志2026，Vol.36Issue(11)：55-62,8.DOI:10.3969/j.issn.1671-7856.2026.11.006
JunB调控C3H10T1/2细胞棕色脂肪细胞分化及产热作用
JunB regulates brown adipocyte differentiation and thermogenesis in mouse C3H10T1/2 cells
摘要
Abstract
Objective We investigated the effect of silencing JunB on the differentiation of C3H10T1/2 cells into brown adipocytes and thermogenic capacity.Methods C3H10T1/2 cells were cultured in vitro and transiently transfected with small interfering RNA(siRNA)to establish a JunB silencing model.The best JunB-siRNA group was selected by Cell Counting Kit-8(CCK-8)and Western blot analysis.Differentiation was induced by a cocktail method,and blank control(Control),induced differentiation model(DM),and induced differentiation model with JunB silencing groups(DM+JunB-siRNA)were created.Lipid droplet formation was analyzed by Oil Red O staining.Western blot analysis and immunofluorescence staining were used to detect uncoupling protein 1(UCP1)and peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α)protein expression,and a JC-1 kit was used to detect mitochondrial membrane potential.Results JunB-siRNA had no significant effect on cell viability(P＞0.05),with JunB-siRNA Ⅰ silencing having the best effect(P＜0.001).JunB silencing increased lipid droplet accumulation in brown adipocytes.UCP1 and PGC-1α expression was up-regulated(P＜0.05,P＜0.001)and mitochondrial membrane potential was enhanced.Conclusions JunB silencing increased the lipid droplet content and thermogenesis of brown adipocytes,likely through improving mitochondrial function.关键词
肥胖/JunB/C3H10T1/2细胞诱导分化/棕色脂肪细胞Key words
obesity/JunB/C3H10T1/2 cells/brown adipocytes分类
生物科学引用本文复制引用
卜丽霞,张苗苗,田嵩浩,和兴敏,郝希望,武玥,赵莹,马明峰..JunB调控C3H10T1/2细胞棕色脂肪细胞分化及产热作用[J].中国比较医学杂志,2026,36(11):55-62,8.基金项目
山西省中医药管理局科研课题(2023ZYYB083) （2023ZYYB083）
山西医科大学汾阳学院科技扶持基金项目(2025A90). （2025A90）
