中国比较医学杂志2026，Vol.36Issue(11)：63-72,10.DOI:10.3969/j.issn.1671-7856.2026.11.007
冻干熊胆粉对干酵母致热模型大鼠的解热作用及机制
Antipyretic effect and mechanism of freeze-dried bear bile powder on rats with yeast-induced fever
摘要
Abstract
Objective This study aimed to evaluate the antipyretic effect of freeze-dried bear bile powder on a rat fever model induced by dry yeast and further elucidate its potential mechanism of action.Methods SPF-grade male SD rats were randomly divided into Control,Model,freeze-dried bear bile powder low-dose group(0.027 g/kg),freeze-dried bear bile powder high-dose group(0.054 g/kg),and aspirin group(0.05 g/kg)using a random number table method.A fever model was established by subcutaneous injection of dry yeast into the back.The differences between body temperature at each time point after modeling and baseline body temperature(△T)were measured and recorded continuously for 8 h.The lung index was calculated.Serum concentrations of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β),interleukin-6(IL-6),5-hydroxytryptamine(5-HT),prostaglandin E2(PGE2),cyclic adenosine monophosphate(cAMP),and cyclooxygenase-2(COX-2)were detected using enzyme-linked immunosorbent assay(ELISA).Histopathological changes in the liver,lung,and hypothalamus were systematically evaluated by hematoxylin-eosin(HE)staining.Meanwhile,the protein expression levels of Toll-like receptor 4(TLR4),myeloid differentiation primary response 88(MyD88),nuclear factor-κB p65(NF-κB p65),phosphorylated NF-κB p65(p-NF-κB p65),and IL-6 in the hypothalamus were quantitatively analyzed by Western blot.Results Compared with the Control group,the Model group showed a significant increase in △T(P＜0.01).The lung index,serum levels of TNF-α,IL-1β,IL-6,COX-2,PGE2,cAMP,and 5-HT,as well as the protein expression levels of TLR4,MyD88,p-NF-κB p65/NF-κB p65,and IL-6 in the hypothalamus,were significantly upregulated(P＜0.05 or P＜0.01).Compared with the model group,both low-and high-dose freeze-dried bear bile powder significantly reduced △T,lung index,serum inflammatory factors TNF-α,IL-1β,and IL-6,and pyrogenic mediators COX-2,PGE2,cAMP,and 5-HT(P＜0.05 or P＜0.01).Western blot results showed that freeze-dried bear bile powder significantly inhibited the protein expression levels of TLR4,MyD88,p-NF-κB p65/NF-κB p65,and IL-6 in the hypothalamus(P＜0.05 or P＜0.01).Histopathological examination showed that both low-and high-dose freeze-dried bear bile powder alleviated dry yeast-induced inflammatory injury in the liver,lung,and hypothalamus.Conclusions Freeze-dried bear bile powder exerted a favorable antipyretic effect on dry yeast-induced fever in rats,and its antipyretic action may be associated with blockade of the TLR4/MyD88/NF-κB signaling cascade.关键词
冻干熊胆粉/解热作用和机制/干酵母/Toll样受体4/核因子-κBKey words
freeze-dried bear bile powder/antipyretic effect and mechanism/dry yeast/Toll-like receptor 4/nuclear factor-κB分类
医药卫生引用本文复制引用
蒋伟,肖建洪,魏荣锐,焦倩,曾林,李庚鑫,祝婧..冻干熊胆粉对干酵母致热模型大鼠的解热作用及机制[J].中国比较医学杂志,2026,36(11):63-72,10.基金项目
江西省重点研发计划-产业链科技创新联合体揭榜挂帅项目(20224BBG71022) （20224BBG71022）
宜春市重点研发计划项目(宜科字[2022]62号). （宜科字[2022]62号）
