中国动物传染病学报2026，Vol.34Issue(2)：63-69,7.
绵羊肺炎支原体盘羊杂交羊感染模型的建立
Establishment of a Argali hybrid Sheep Model of Mycoplasma Ovipneumoniae Infection
摘要
Abstract
To establish an infection model of Mycoplasma ovipneumoniae(MO)in argali crossbred sheep,strain MO-XJ15 isolated from lung tissues of diseased sheep was identified as a virulent strain via immunofluorescence assay and chicken embryo inoculation.The strain was serially passaged three times in lambs.Lesioned lung tissues from infected sheep were collected,homogenized and filtered to prepare tissue-derived virulent inoculum.The MO load in the inoculum was quantified by quantitative real-time PCR(qPCR).Twenty-four MO-susceptible argali crossbred sheep were randomly allocated into four groups(n=6 per group).Each animal received an intratracheal inoculation of 5 mL undiluted virulent inoculum,1:10 diluted inoculum,1:100 diluted inoculum,or phosphate-buffered saline(PBS),respectively.After inoculation,clinical signs were evaluated using a standardized scoring system,and thoracic fluoroscopy was conducted.All sheep were necropsied on day 29 post-infection.Gross pulmonary lesions were examined and scored with ImageJ software.Lung tissue samples were collected for hematoxylin-eosin(HE)staining and histopathological assessment.The MO load in the tissue-derived virulent inoculum was 1.8×105 copies/mL.Typical clinical manifestations and pathological lesions of ovine mycoplasmal pneumonia were observed in all sheep in the undiluted group and several animals in the 1:10 dilution group.In conclusion,intratracheal inoculation with tissue-derived virulent inoculum of strain MO-XJ5 containing MO at a load of≥1.8×105 copies/mL can successfully establish an ovine mycoplasmal pneumonia model in argali crossbred sheep.关键词
绵羊肺炎支原体/疾病模型/肺脏病变评分/临床症状评分Key words
Mycoplasma ovipneumoniae/disease models/lung lesion score/clinical sign score分类
农业科技引用本文复制引用
高之煜,魏立翔,曹鑫艳,张彦兵,孙延鸣..绵羊肺炎支原体盘羊杂交羊感染模型的建立[J].中国动物传染病学报,2026,34(2):63-69,7.基金项目
国家自然科学基金项目(32060133,32360901) （32060133,32360901）
新疆维吾尔自治区自然科学基金资助项目(2022D01B197) （2022D01B197）
