中国动物传染病学报2026，Vol.34Issue(2)：108-114,7.
猪圆环病毒2型质粒标准物质研制
Preparation of Reference Material for Genomic Plasmid of Porcine Circovirus Type 2
摘要
Abstract
The reference material(RM)for Porcine circovirus type 2(PCV2)is not available currently in China.In this study,RM containing genomic plasmids of PCV2 strain 08TJ was prepared using molecular biology technology.A digital PCR method was established and used to evaluate the homogeneity,stability and characteristics of the RM.Meanwhile,trials of the RM were performed for applications.The homogeneity evaluation showed that there was no statistical difference in within batch and between batch,indicating that the RM we prepared was homogeneous.The monitoring of stability revealed that the RM was stable when it was stored at different temperatures for different time periods,including-20℃for 12 months,4℃for 120 days,25℃for 14 days,and at 37℃for 9 days.Moreover,the RM was stable after it was treated for 10 freezing-thawing cycles.The reference value of the RM was(1.1±0.2)×104 copies/µL.The result of tria l experiments demonstrated that the RM had good commutability with field samples.In conclusion,the RM we prepared had accurate value,good homogeneity and stability.The RM described in this study enhanced the capability of PCV2 detection in veterinary laboratories in China.关键词
猪圆环病毒/标准物质/均匀性/稳定性Key words
Porcine circovirus/reference material/homogeneity/stability分类
农业科技引用本文复制引用
张媛,周智,倪建强,孙雨,李琦,翟新验,刘玉良,王传彬,顾小雪,毕一鸣,徐琦,原霖,高旭年,李尔华,钟凤然..猪圆环病毒2型质粒标准物质研制[J].中国动物传染病学报,2026,34(2):108-114,7.基金项目
科技基础性工作专项(2013YF113300) （2013YF113300）
