中国烟草科学2026，Vol.47Issue(3)：18-29,12.DOI:10.13496/j.issn.1007-5119.2026.03.003
光叶紫花苕还田对丽江烟田土壤微生态和烤烟品质的影响
Effects of Vicia villosa Green Manure Incorporation on Soil Microecology and Flue-Cured Tobacco Quality in Lijiang
摘要
Abstract
The study aims to investigate the effects of planting Vicia villosa green manure during winter fallow and its incorporation into soil on soil environment of tobacco fields and flue-cured tobacco quality in Lijiang,Yunnan Province.Four sowing rate treatments were established:(D1)60 kg/hm2,(D2)45 kg/hm2,(D3)30 kg/hm2,and(CK)0 kg/hm2;corresponding to the incorporation rates of 7 800,6 300,3 800 and 0 kg/hm2,respectively.Rhizosphere soil nutrients and microbial communities,as well as yield and quality of flue-cured tobacco leaves were assessed 70 days after transplantation.Results showed that the incorporation treatment of 7 800 kg/hm2 significantly increased soil electrical conductivity(increase of 48.83%),available nitrogen(67.41%),available phosphorus(98.61%),available potassium(104.76%),and organic matter(24.11%).Microbiome analysis revealed that the high application rates(7 800 and 6 300 kg/hm2)significantly enhanced the bacterial α-diversity but not fungal α-diversity.Vicia villosa incorporation significantly altered microbial community structure,and enriched of beneficial microorganisms,including Sphingobacterium,Terrimonas,Metarhizium,and Penicillium,involved in nutrient activation and disease suppression,was significantly increased.Redundancy analysis(RDA)indicated that soil physicochemical properties explained 38.09%and 37.85%of the variation in bacterial and fungal communities,respectively,with available nitrogen as the main driving factor.Co-occurrence network analysis revealed that the incorporation induced reconstruction of microbial functional modules,enriching bacterial modules that dominated nitrogen cycling,photosynthesis,and organic matter degradation,while significantly reducing the abundance of fungal modules with potential negative functions.Moreover,changes in all modules were significantly correlated with soil physicochemical properties.Agro-economic analysis showed that the application of 7 800 kg/hm2 dry mass(equivalent to 26 441 kg/hm2 fresh mass)achieved optimal yield,along with significantly enhanced appearance&sensory quality of tobacco leaves.The study confirms that green manure-driven rhizosphere micro-ecosystem reconstruction represents an effective approach to improve flue-cured tobacco quality,and Vicia villosa has promising applications in green tobacco production.关键词
烤烟/绿肥/微生物/多样性Key words
flue-cured tobacco/green manure/microorganisms/diversity分类
农业科技引用本文复制引用
余飞,曲思远,沈嘉,祖朝龙,汪季涛,尹乐,刘非,梁昊,周初跃,李兰周,朱一帆,尹浩,廖德智..光叶紫花苕还田对丽江烟田土壤微生态和烤烟品质的影响[J].中国烟草科学,2026,47(3):18-29,12.基金项目
安徽中烟工业有限责任公司基地技术服务项目(2023340000340030) （2023340000340030）
