中国烟草科学2026，Vol.47Issue(3)：53-61,9.DOI:10.13496/j.issn.1007-5119.2026.03.007
粘烟草蔗糖酯的分离鉴定及其抑菌和抗TMV活性
Isolation,Identification,and Evaluation of the Antifungal and Anti-TMV Activities of Sucrose Esters from Nicotiana glutinosa
摘要
Abstract
This study investigated the potential application of sucrose esters(SEs)derived from Nicotiana glutinosa for biopesticide development.SEs were extracted using solvent extraction,purified by normal-phase silica gel column chromatography,followed by identification and quantification using GC/MS analysis.The antifungal properties against phytopathogenic fungi was evaluated through the mycelial growth rate method,and the anti-tobacco mosaic virus(TMV)activity using the half-leaf lesion assay.Two distinct SEs configurations were isolated and identified from N.glutinosa:SE-Ⅱ(acetylated at fructose C-3')and SE-Ⅲ(non-acetylated at fructose C-3').SEs contents varied significantly among tissues,with the highest levels observed in upper leaves(3.56 mg/g fresh weight)and flowers(3.21 mg/g),substantially exceeding those in middle leaves,lower leaves,and stems.The ratio of SE-Ⅱ to SE-Ⅲ in various organs decreases with the rising position on the tobacco plant.The crude SEs extract,SE-Ⅱ,and SE-Ⅲexhibited broad-spectrum antifungal activity,with notably strong inhibition observed against Botrytis cinerea.At a concentration of 20 μg/mL,three SEs forms achieved inhibition rates of 57.75%,65.58%,and 84.73%,respectively.The antifungal activity of the crude SEs extract against B.cinerea was superior to the botanical fungicides eugenol and osthole.SE-Ⅱ showed comparable activity to the synthetic fungicide pyrimethanil,whereas SE-Ⅲ exhibited significantly stronger inhibitory effects than pyrimethanil.In anti-TMV assays at 200 μg/mL,inhibition rates were 36.33%(crude extract),69.09%(SE-Ⅱ),and 64.41%(SE-Ⅲ).The antiviral activity of the crude SEs extract matched that of oligosaccharins,while SE-Ⅱ and SE-Ⅲ showed efficacy comparable to that of ningnanmycin.These findings identify N.glutinosa represents a valuable source of diverse and bioactive SEs showing promising potential for the management of biofungicides against gray mold and antiviral agents for crop protection.关键词
粘烟草/蔗糖酯/分离鉴定/抑菌活性/抗TMV活性Key words
Nicotiana glutinosa/sucrose esters/isolation and identification/antifungal activity/anti-TMV activity分类
轻工纺织引用本文复制引用
杨泽宇,桂祖卿,胡棕棂,赵兴能,张鹏,韩晓,杜咏梅..粘烟草蔗糖酯的分离鉴定及其抑菌和抗TMV活性[J].中国烟草科学,2026,47(3):53-61,9.基金项目
中国烟草总公司重点研发项目(110202402033) （110202402033）
中国农业科学院科技创新工程项目(APTIP-TRIC05) （APTIP-TRIC05）
