中国烟草科学2026，Vol.47Issue(3)：73-81,92,10.DOI:10.13496/j.issn.1007-5119.2026.03.009
不同工业可用性上部烟叶的差异标志物及其通路分析
Identification of Differential Markers and Pathway Analysis of Upper Tobacco Leaves with Different Industrial Usability
摘要
Abstract
To elucidate the causes of industrial usability differences in upper tobacco leaves,this study employed untargeted metabolomics to analyze upper flue-cured tobacco samples of varying industrial usability from three aroma types:robust,medium,and fresh.We identified differential biomarkers,their enriched pathways,and analyzed their relationship with sensory quality.The results showed that all upper tobacco leaf samples used in the experiment were classified as Class I or Class II industrial usability,with no Class III samples.Sugars,lipids,amino acids,organic acids,and alkaloids constituted a high proportion of compounds in the three aroma types of tobacco leaves.Based on the criteria of VIP≥1.0 and(FC<0.5 or FC>2.0),five and seven differential biomarkers were screened for robust and fresh aroma types with different industrial usability in 2022,while ten and four differential biomarkers were identified for 2023,respectively.These biomarkers belonged to lipids,amino acids,alkaloids,organic acids,sugars,and phenolic compounds.In the robust aroma producing region's upper tobacco leaves from both 2022 and 2023,L-glutamine was a common differential biomarker,whereas no common differential biomarkers were found in the fresh aroma type.Further analysis of the correlation between differential biomarkers and sensory quality revealed that the accumulation of amino acids,lipids,and phenolic compounds positively regulated the industrial usability of upper tobacco leaves in the robust aroma producing region,while the accumulation of lipids and amino acids negatively regulated the industrial usability of upper tobacco leaves in the fresh aroma producing region.Differential metabolite enrichment pathway analysis indicated that the 26 differential biomarkers identified in this study were significantly and primarily enriched in amino acid metabolism-related pathways.L-glutamine is likely a core differential biomarker influencing the industrial usability of robust aroma tobacco leaves.关键词
烤烟/工业可用性/非靶向代谢组学/差异标志物/富集通路Key words
flue-cured tobacco/industrial usability/untargeted metabolomics/differential biomarkers/enriched pathways分类
轻工纺织引用本文复制引用
严寒,张翔,李将,王建伟,刘相甫,杨丙烨,岳彩鹏,赵才能..不同工业可用性上部烟叶的差异标志物及其通路分析[J].中国烟草科学,2026,47(3):73-81,92,10.基金项目
中国烟草总公司重点科技项目(110202102035) （110202102035）
中国烟草总公司福建省公司科技项目(2023350000200084) （2023350000200084）
江苏中烟工业有限责任公司科技项目(D2023088) （D2023088）
