中国烟草科学2026，Vol.47Issue(3)：129-142,14.DOI:10.13496/j.issn.1007-5119.2026.03.015
烟草中萜类化合物的合成、调控与代谢工程研究进展
Advances in the Synthesis,Regulation,and Metabolic Engineering of Terpenoids in Tobacco
摘要
Abstract
Terpenoids possess important application value in the pharmaceutical and chemical industries.Tobacco,primarily Nicotiana benthamiana and Nicotiana tabacum,has emerged as an ideal plant chassis for the synthesis of high-value terpenoids.This paper systematically reviews the types and properties of terpenoids in tobacco,elucidates their biosynthetic pathways and key enzymes,and discusses the complex regulatory network composed of transcription factors,phytohormones,and environmental factors.A special focus is placed on summarizing metabolic engineering strategies for using tobacco as a bioreactor,including gene expression regulation via transient expression and stable transformation,enzyme modification and subcellular localization,and product storage and secretion.Representative case studies are analyzed to demonstrate their application in enhancing the yields of target terpenoids.Finally,the current research challenges are analyzed,such as incompletely characterized metabolic networks,insufficient biosynthetic elements,and difficulties in balancing terpenoid yield with plant growth.Prospects for future research directions are outlined,including multi-omics integration,development of synthetic biology toolkits,construction of universal chassis,and industrial-scale deployment.This review aims to provide a theoretical reference for the innovative and high-value utilization of tobacco resources.关键词
烟草/萜类化合物/生物合成/代谢工程/多用途利用Key words
tobacco/terpenoids/biosynthesis/metabolic engineering/multi-purpose utilization分类
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张鑫,汪阳忠,戴泽元,田晨菲,韩明辰,王静,徐玮杰,谢雯燕,杨凯..烟草中萜类化合物的合成、调控与代谢工程研究进展[J].中国烟草科学,2026,47(3):129-142,14.基金项目
上海市科技计划项目(24HC2820400) （24HC2820400）
上海烟草集团有限责任公司科技项目(K2023-1-002P) （K2023-1-002P）
