中国中医药信息杂志2026，Vol.33Issue(6)：38-45,8.DOI:10.19879/j.cnki.1005-5304.202511626
基于网络药理学和动物实验探讨生腺布液汤治疗干燥综合征的作用机制
Exploration on the Mechanism of Shengxian Buye Decoction in Treating Sjögren's Syndrome Based on Network Pharmacology and Animal Experiments
摘要
Abstract
Objective To explore the mechanism of Shengxian Buye Decoction in the treatment of Sjögren's syndrome(SS)using network pharmacology;To conduct animal experiments for validation.Methods TCMSP,HERB and TCMID databases were used to retrieve and screen the active components and corresponding targets of Shengxian Buye Decoction.The core components were obtained through the drug-component-target network,and the SS disease targets were retrieved from GeneCards,OMIM,TTD,PharmGKB,and DrugBank databases.The protein-protein interaction network was constructed and the core targets were screened.The intersection targets were enriched by GO and KEGG pathways.A total of 24 SS model NOD mice were randomly divided into the Chinese materia medica group,Western medicine group,and model group,with 8 mice in each group;another 8 BALB/c mice were selected as the normal group.The mice in each group were intragastrically administered with Shengxian Buye Decoction(9.88 g/kg),hydroxychloroquine sulfate solution(0.052 g/kg),and an equal volume of distilled water,respectively,for 8 consecutive weeks.The water intake was recorded at 1,4,and 8 weeks.HE staining was employed to observe the pathological changes of the submandibular gland tissue.ELISA was used to compare the serum levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α).Western blot was used to detect the protein expression of PI3K-Akt pathway.Results Through network pharmacology screening,the top 7 core active components of Shengxian Buye Decoction for treating SS were quercetin,kaempferol,luteolin,β-sitosterol,stigmasterol,formononetin and wogonin,with 101 corresponding therapeutic targets.The top 5 targets were IL6,IL1B,TNF,AKT1,and MMP9.These key targets were mainly enriched in the PI3K-Akt,MAPK,and TNF signaling pathways.Animal experiment results showed that,compared with the model group,the water intake of mice in the Chinese materia medica group and Western medicine group significantly decreased at 4 and 8 weeks after treatment(P<0.05);focal lymphoid cell aggregation in the submandibular gland tissue of mice decreased;the serum contents of inflammatory factors IL-6 and TNF-α were significantly reduced(P<0.05);the expressions of p-PI3K,PI3K,p-Akt,and Akt proteins of the submandibular gland were up-regulated(P<0.05).Conclusion Shengxian Buye Decoction may exert anti-inflammatory and immune-regulating effects to treat SS by regulating signaling pathways such as PI3K-Akt,MAPK,and TNF.关键词
干燥综合征/生腺布液汤/网络药理学/实验验证Key words
Sjögren's syndrome/Shengxian Buye Decoction/network pharmacology/experimental Validation分类
医药卫生引用本文复制引用
王金娥,张正国,刘龙霞,张寒,侯小双,罗亚萍,靳可心,张莹..基于网络药理学和动物实验探讨生腺布液汤治疗干燥综合征的作用机制[J].中国中医药信息杂志,2026,33(6):38-45,8.基金项目
河北省重点研发计划(192777123D) （192777123D）
河北省老年病防治项目(2019064577) （2019064577）
政府资助临床医学优秀人才项目(ZF2025296) （ZF2025296）
