中国中医药信息杂志2026，Vol.33Issue(6)：82-89,8.DOI:10.19879/j.cnki.1005-5304.202511708
基于IL-6/JAK2/STAT3通路探讨化浊解毒方对5/6肾切除大鼠肾纤维化的影响
Exploration on the Effects of Huazhuo Jiedu Prescription on Renal Fibrosis in 5/6 Nephrectomized Rats Based on the IL-6/JAK2/STAT3 Signaling Pathway
摘要
Abstract
Objective To investigate the effects of Huazhuo Jiedu Prescription on renal injury and the IL-6/JAK2/STAT3 pathway in rats with chronic renal failure(CRF);To explore its mechanism in delaying renal fibrosis.Methods Totally 61 male SD rats were randomly divided into a normal group(n=10)and a modeling group(n=51).CRF model was established via 5/6 nephrectomy.The successful modeled rats were randomly divided into model group,losartan potassium group(4.5 mg/kg)and Huazhuo Jiedu Prescription low-,medium-and high-dosage groups(1.175,2.350 and 4.700 g/kg),with 8 rats in each group.Each treatment group was given corresponding drugs by gavage once a day for 8 weeks.Renal histopathology was examined using HE and Masson staining,serum contents of TNF-α,IL-6 and MCP-1 were detected by ELISA,the expressions of Col-Ⅰ,Col-Ⅲ,α-SMA,TGF-β1,Bcl-2,Bax,Cleaved Caspase-3,IL-6,JAK2,STAT3,p-JAK2,p-STAT3 were detected by Western blot,renal apoptosis was assessed using TUNEL staining,the positive expressions of Col-Ⅰ,Col-Ⅲ,IL-6,p-JAK2 and p-STAT3 were detected by immunohistochemistry.Results Compared with the normal group,the renal tissue structure of rats in the model group exhibited significant damage,with an aggravated degree of fibrosis,the contents of TNF-α,IL-6 and MCP-1 in serum and the protein expressions of Col-Ⅰ,Col-Ⅲ,α-SMA,TGF-β1,Bax,Cleaved Caspase-3,IL-6,p-JAK2 and p-STAT3 in renal tissue significantly increased(P<0.01),while the protein expression of Bcl-2 significantly decreased(P<0.01).Compared with the model group,the renal tissue damage was alleviated to varying degrees,and the area of collagen fibers decreased in each administration group,the contents of TNF-α,IL-6 and MCP-1 in serum and the protein expressions of Col-Ⅰ,Col-Ⅲ,α-SMA,TGF-β1,Bax,Cleaved Caspase-3,IL-6,p-JAK2 and p-STAT3 in renal tissue of rats in Huazhuo Jiedu Prescription medium-and high-dosage groups and losartan potassium groups significantly decreased(P<0.05,P<0.01),and the protein expression of Bcl-2 significantly increased(P<0.05,P<0.01).The expression of Col-Ⅰ,Col-Ⅲ,IL-6,p-JAK2 and p-STAT3 were consistent with Western blot.Conclusion Huazhuo Jiedu Prescription may alleviate renal injury and delay renal fibrosis by regulating the IL-6/JAK2/STAT3 pathway,inhibiting inflammation,and modulating apoptosis.关键词
化浊解毒方/慢性肾衰竭/肾纤维化/凋亡/IL-6/JAK2/STAT3通路/大鼠Key words
Huazhuo Jiedu Prescription/chronic renal failure/renal fibrosis/apoptosis/IL-6/JAK2/STAT3 pathway/rats分类
医药卫生引用本文复制引用
邓婉莹,郑琳琳,高卉,高芸慧,邹佳颖,边东,冯忖,魏晓娜..基于IL-6/JAK2/STAT3通路探讨化浊解毒方对5/6肾切除大鼠肾纤维化的影响[J].中国中医药信息杂志,2026,33(6):82-89,8.基金项目
河北省自然科学基金(H2023423067) （H2023423067）
河北省中医药管理局科研计划(2025206) （2025206）
