种子2026，Vol.45Issue(5)：177-184,8.DOI:10.16590/j.cnki.1001-4705.2026.05.177
巴戟天种质复壮组织培养快繁技术体系研究
Study on Tissue Culture-based Rapid Propagation System for Germplasm Rejuvenation of Morinda officinalis
摘要
Abstract
In order to establish a tissue culture rapid propagation technology system for the rejuve-nation of the medicinal plant Morinda officinalis,using the young shoot tips of the main cultivated variety Heisuizai in Zhaoqing as the explants,the effects of MS and N6 culture media and different concentrations of 6-BA and IBA combinations on their induction,differentiation,proliferation and rooting were studied.The most suitable culture media at each developmental stage were screened.The results showed that the MS medium had the best induction effect.The initial generation bud induction medium was MS+IBA 0.10 mg/L+6-BA 1.50 mg/L.The secondary generation proliferation medium was MS+6-BA 0.5-1.00 mg/L+IBA 0.10 mg/L.The survival rate of adventitious buds was 95.56％-98.23％,and the number oftillers was 6.97-7.66.The vigorous seedling culture medium was MS+6-BA 0.30 mg/L+IBA 0.10 mg/L.The rooting culture medium was MS+1.00 g/L activated carbon+1.00 mg/L IBA.The rooting rate was 95.75％,and the rooting coefficient was 11.17.After the o-riginal bottle of the tissue culture seedlings was acclimatized for 15 days,which were transplanted to peat soil or peat soil+river sand substrate.The survival rate reached 98.33％after 70 days and the growth was vigorous.关键词
巴戟天/种质复壮/茎尖/基本培养基/快繁技术Key words
Morinda officinalis/germplasm rejuvenation/shoot tip/basal medium/rapid propagation分类
农业科技引用本文复制引用
邵玲,叶光旺,廖舒婷,黄绮婷,吴雅琪..巴戟天种质复壮组织培养快繁技术体系研究[J].种子,2026,45(5):177-184,8.基金项目
广东省教育厅乡村振兴重点领域研究项目(2023ZDZX4063) （2023ZDZX4063）
广东省科学技术厅2025年中央引导地方科技发展专项(2025B0202010052) （2025B0202010052）
广东省科学技术厅"百千万工程"农村科技特派员第二轮重点派驻项目(KTP20240673) （KTP20240673）
广东省科技创新战略专项资金(pdjh2024b407) （pdjh2024b407）
肇庆学院大学生创新创业训练计划项目(X202510580094X) （X202510580094X）
