种子2026，Vol.45Issue(5)：184-190,7.DOI:10.16590/j.cnki.1001-4705.2026.05.184
堇叶碎米荠高效离体再生体系构建与优化
Establishment and Optimization of an Efficient in Vitro Regeneration System for Cardamine circaeoides
摘要
Abstract
To establish an efficient in vitro regeneration system for Cardamine circaeoides,the seeds of Cardamine circaeoides were used as the material for obtaining sterile tissue culture seedlings.Based on the MS medium,by adjusting the types and concentrations of plant growth regulators,the effects of differences in explant sites and hormone ratios on the formation of callus,the differentiation of ad-ventitious buds,and the induction of rooting were studied.The results showed that the best disinfec-tion method for the seeds was 0.1％HgCl2 for 7 minutes;the best explant for inducing callus was the hypocotyl,and the best induction medium was MS+6-BA0.5 mg/L+NAA0.1 mg/L,with an induc-tion rate of 75.93％.The best medium formula for the differentiation of adventitious buds was MS+6-BA 1.0 mg/L+NAA0.5 mg/L,with a differentiation rate of 85.93％.The rooting medium was 1/2 MS+IBA 1.0 mg/L,with a rooting rate of 98.52％.After the tissue culture seedlings were transplan-ted to the substrate,they grew fast and robustly,and the transplant survival rate reached 85％.关键词
堇叶碎米荠/组织培养/高效离体再生体系/植物生长调节剂/愈伤组织Key words
Cardamine circaeoides/tissue culture/highly efficiency in vitro culture/plant growth regulators/callus分类
农业科技引用本文复制引用
康宇,明佳佳,王国贵,殷红清,向极钎,朱云芬..堇叶碎米荠高效离体再生体系构建与优化[J].种子,2026,45(5):184-190,7.基金项目
恩施州启航专项科技计划项目"堇叶碎米荠高效离体再生体系研究"(2024027) （2024027）
湖北省重大科技项目"有机硒生物合成及产业化关键技术研究"(2024BBA002) （2024BBA002）
湖北省支持种业高质量发展资金项目(HBZY2023B004-7) （HBZY2023B004-7）
恩施州重大科技项目(Z20250001) （Z20250001）
