针灸临床杂志2026，Vol.42Issue(6)：67-74,8.DOI:10.19917/j.cnki.1005-0779.026111
眶内电针通过调控CASR介导的NLRP3炎症小体活化促进大鼠动眼神经损伤修复的机制研究
Mechanistic Study of Intraorbital EA in Promoting Repair of Oculomotor Nerve Injury in Rats via Regulation of CASR-Mediated NLRP3 Inflammasome Activation
摘要
Abstract
Objective:To observe the effects of intraorbital electro-acupuncture(EA)on neurological function in rat model of oculomotor nerve injury and to explore its underlying mechanisms in oculomotor nerve functional reconstruction from the perspective of inflammatory responses.Methods:90 SD rats were randomly divided into the sham operation group,the model group,the EA group,the inhibitor group,and the EA plus inhibitor group,with 18 rats in each group.Each group was further subdivided into three time-point subgroups(3,7,and 14 days),with 6 rats in each subgroup.An oculomotor nerve injury model was established using the intraorbital clamping method.In the EA group,EA was applied to the superior rectus,inferior rectus,and medial rectus muscles for 20 min per day for 14 days.General conditions and oculomotor nerve function(including pupil diameter and ocular deviation distance)were assessed.HE staining was performed to observe the morphological changes of the oculomotor nerve.Western blotting was used to determine the protein expressions of CASR,NLRP3,IL-1β,and IL-18,while RT-qPCR was employed to measure the mRNA expressions of ASC,Caspase-1,and related factors.Results:Compared with the sham operation group,the model group showed significant differences in general conditions,oculomotor nerve function,and the expressions of related factors(P＜0.01).Compared with the model group,the EA group showed improvement in inflammatory markers only at 3 days post-treatment(P＜0.05),while at 7 and 14 days post-treatment,all assessed indicators were significantly improved(P＜0.01).In the inhibitor group,all indicators at each time point were significantly different compared with the model group(P＜0.05,P＜0.01).Compared with the EA group,at 3 days post-treatment,the EA plus inhibitor group showed no significant difference in general conditions(P＞0.05),while all other indicators at subsequent time points were significantly improved(P＜0.01).HE staining revealed disorganized nerve fibers and demyelination in the model group,whereas the treatment groups demonstrated remyelination and structural restoration of the oculomotor nerve.Conclusion:Intraorbital EA may promote repair of oculomotor nerve injury in rats by regulating CASR-mediated NLRP3 inflammasome activation.关键词
动眼神经损伤/CASR/眶内电针/NLRP3/修复机制Key words
Oculomotor nerve injury/CASR/Intraorbital electro-acupuncture/NLRP3/Repair mechanism分类
医药卫生引用本文复制引用
方姗姗,李元,姚冰,刘同岩,周凌云..眶内电针通过调控CASR介导的NLRP3炎症小体活化促进大鼠动眼神经损伤修复的机制研究[J].针灸临床杂志,2026,42(6):67-74,8.基金项目
国家自然科学基金(82074524,81674052). （82074524,81674052）
