针灸临床杂志2026，Vol.42Issue(6)：75-82,8.DOI:10.19917/j.cnki.1005-0779.026112
针刺联合姜黄素调控Shh/Gli1通路对慢性萎缩性胃炎大鼠胃粘膜损伤的影响
Effects of Acupuncture Combined with Curcumin on Gastric Mucosal Injury in Rats with CAG via Regulation of Shh/Gli1 Pathway
摘要
Abstract
Objective:To investigate the effects of acupuncture combined with curcumin(Cur)on gastric mucosal injury in rats with chronic atrophic gastritis(CAG)through regulation of the Sonic hedgehog/glioma-associated oncogene homolog 1(Shh/Gli1)pathway.Methods:The rat model of CAG was established,and the successfully modeled rats were randomly divided into the CAG group,the acupuncture group,the Cur group,the Cur+acupuncture group,and the Cur+acupuncture+cyclopamine(Shh/Gli1 pathway inhibitor)group,with 15 rats in each group.In addition,15 normal rats were randomly selected as the NC group.The expression levels of serum inflammation-related factors and gastric function-related indicators were measured.Histopathological changes in gastric tissues were examined.The ultrastructure of gastric mucosal cells was observed.Apoptosis of gastric mucosal cells was assessed.Immunohistochemistry was used to detect the expression of apoptosis-related proteins in gastric tissues,and Western blot was performed to determine the expression of Shh/Gli1 pathway-related proteins in gastric tissues.Results:Compared with the NC group,rats in the CAG group showed thinning of the gastric mucosa,glandular atrophy,loose and disordered glandular arrangement,marked inflammatory cell infiltration,abnormal mitochondrial structure,and sparse and fractured cristae;in addition,the levels of IL-6,IL-8,TNF-α,motilin(MTL),apoptosis rate,and Bax were significantly increased,whereas the levels of gastrin(GAS),somatostatin(SS),pepsinogen(PP),Bel-2,Shh,and Gli1 were significantly decreased(P＜0.05).Compared with the CAG group,rats in the acupuncture group,the Cur group,and the Cur+acupuncture group showed improved gastric mucosal injury,reduced inflammatory cell infiltration,and restored mitochondrial structure;moreover,the levels of IL-6,IL-8,TNF-α,MTL,apoptosis rate,and Bax were significantly decreased,whereas the levels of GAS,SS,PP,Bel-2,Shh,and Gli1 were significantly increased(P＜0.05).Compared with the acupuncture group and the Cur group,rats in the Cur+acupuncture group exhibited milder gastric mucosal injury,less inflammatory cell infiltration,and less mitochondrial structural damage;the levels of IL-6,IL-8,TNF-α,MTL,apoptosis rate,and Bax were significantly lower,whereas the levels of GAS,SS,PP,Bcl-2,Shh,and Gli1 were significantly higher(P＜0.05).Compared with the Cur+acupuncture group,rats in the Cur+acupuncture+cyclopamine group showed aggravated gastric mucosal injury and inflammatory cell infiltration,mitochondrial swelling,and abnormal mitochondrial structure;the levels of IL-6,IL-8,TNF-α,MTL,apoptosis rate,and Bax were significantly increased,whereas the levels of GAS,SS,PP,Bcl-2,Shh,and Gli1 were significantly decreased(P＜0.05).Conclusion:Acupuncture combined with curcumin can improve gastric function in rats with CAG and exert a protective effect against gastric mucosal injury,which may be closely related to activation of the Shh/Gli1 pathway.关键词
慢性萎缩性胃炎/针刺/姜黄素/音猬因子/胶质瘤相关癌基因同源物通路/胃粘膜损伤Key words
Chronic atrophic gastritis/Acupuncture/Curcumin/Sonic hedgehog/glioma associated oncogene homolog 1 pathway/Gastric mucosal damage分类
医药卫生引用本文复制引用
李翻红,李仁洁,毛维武..针刺联合姜黄素调控Shh/Gli1通路对慢性萎缩性胃炎大鼠胃粘膜损伤的影响[J].针灸临床杂志,2026,42(6):75-82,8.基金项目
甘肃省自然科学基金(GSTSKY-031). （GSTSKY-031）
