针灸临床杂志2026，Vol.42Issue(6)：83-89,7.DOI:10.19917/j.cnki.1005-0779.026113
夹脊穴埋线联合跑台训练对脊髓损伤大鼠脊髓神经修复及运动功能的影响
Effects of Jiaji Acupoints Catgut Embedding Combined with Treadmill Training on Spinal Cord Neural Repair and Motor Function in Rats with SCI
摘要
Abstract
Objective:To investigate the effects and underlying mechanisms of Jiaji acupoints catgut embedding(AE)combined with treadmill training(TM)on spinal cord neural repair and motor function in rats with spinal cord injury(SCI).Methods:The SCI model was established using the modified Allen's method.The successfully modeled rats were randomly divided into the SCI group,the AE group,the TM group,and combined group(AE+TM)group,with an additional sham operation(Sham)group,with 10 rats in each group.Rats in the AE,TM,and AE+TM groups received respective interventions for 4 continuous weeks.Behavioral changes were assessed using the Basso-Beattie-Bresnahan(BBB)locomotor rating scale,inclined plane test,and footprint analysis.Spinal cord nerve conduction velocity was measured using a multichannel electrophysiological recorder.Histopathological changes and neuronal survival in spinal cord tissue were observed using HE staining and Nissl staining.Levels of IL-1β,IL-6,and TNF-α in spinal cord tissue were determined by ELISA.Western blot analysis was used to detect the protein expressions of related to the brain-derived neurotrophic factor(BDNF)/tropomyosin receptor kinase B(TrkB)signaling pathway in spinal cord tissue.Results:Compared with the Sham group,rats in the SCI group showed significantly decreased BBB scores,inclined plane angles,and hindlimb stride length and stride width(P＜0.05);the levels of IL-1 β,IL-6,and TNF-α in spinal cord tissue were significantly increased(P＜0.05),while nerve conduction velocity,neuronal survival,BDNF protein expression,and the p-TrkB/TrkB protein ratio were significantly decreased(P＜0.05).Compared with the SCI group,rats in the AE,TM,and AE+TM groups exhibited significantly increased BBB scores,inclined plane angles,and hindlimb stride length and stride width(P＜0.05);the levels of IL-1β,IL-6,and TNF-α in spinal cord tissue were significantly decreased(P＜0.05),whereas nerve conduction velocity,neuronal survival,BDNF protein expression,and the p-TrkB/TrkB protein ratio were significantly increased(P＜0.05).The AE+TM group showed significantly greater improvements in spinal cord neural repair and motor function than the AE group and the TM group(P＜0.05).Conclusion:AE combined with TM promotes spinal cord neural repair and improves motor function in rats with SCI,and its mechanism may be related to activation of the BDNF/TrkB signaling pathway.关键词
脊髓损伤/夹脊穴埋线/跑台训练/神经修复/脑源性神经营养因子/原肌球蛋白相关激酶BKey words
Spinal cord injury/Jiaji acupoints catgut embedding/Treadmill training/Neural repair/BDNF/TrkB分类
医药卫生引用本文复制引用
马晓飞,戴媛,盛浩,朱元元,刘易,张朝驹,蔡青..夹脊穴埋线联合跑台训练对脊髓损伤大鼠脊髓神经修复及运动功能的影响[J].针灸临床杂志,2026,42(6):83-89,7.基金项目
湖北省自然科学基金联合基金项目(2024AFD348) （2024AFD348）
荆州市科技计划项目(指导性)(2024HD166) （指导性）
荆州市儿童青少年脊柱侧弯中医药干预试点地区建设项目(国中药综结[20241189号]) （国中药综结[20241189号]）
荆州市中医药研究所自主选题项目(ZZXT2024Y07). （ZZXT2024Y07）
