针灸推拿医学（英文版）2026，Vol.24Issue(3)：213-228,16.DOI:10.1007/s11726-026-1572-y
针刺对抑郁症模型大鼠海马miR-152-3p、miR-145-5p及CaMKⅡ/NF-κB信号通路的影响
Effects of acupuncture on miR-152-3p,miR-145-5p,and CaMKⅡ/NF-κB signaling pathway in hippocampus of depression model rats
摘要
Abstract
Objective:To observe the effects of the governor vessel-unblocking and mind-regulating acupuncture method on miR-152-3p,miR-145-5p,key molecules of the calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ)/nuclear factor(NF)-κB signaling pathway,and downstream inflammatory factors in cornu ammonis area 1(CA1)in chronic unpredictable mild stress(CUMS)model rats,thereby exploring the possible mechanism of the governor vessel-unblocking and mind-regulating acupuncture method in treating depression. Methods:Twelve of 48 Sprague-Dawley rats were randomly selected as a blank control group,and the other rats were subjected to CUMS for 28 consecutive days to prepare a depression rat model.The successful model rats were randomly divided into a model group,an acupuncture group,and a fluoxetine group,with 12 rats in each group.The blank control and model groups did not receive intervention.The acupuncture group received acupuncture at Baihui(GV20),Shuigou(GV26),Shenting(GV24),and Dazhui(GV14),6 d a week for a total of 4 weeks;the fluoxetine group was administered fluoxetine by gavage for 28 consecutive days.The behavioral changes of rats in each group after intervention were observed;hematoxylin-eosin staining and Nissl staining were used to observe the pathological morphology and neuronal damage of the hippocampal CA1 region.The levels of interleukin(IL)-1β,IL-6,tumor necrosis factor(TNF)-α,IL-10,and 5-hydroxytryptamine(5-HT)in the hippocampus and serum were detected by enzyme-linked immunosorbent assay.Relative expression levels of miR-152-3p,miR-145-5p,and mRNAs of CaMKⅡ and NF-κB p65 in the hippocampus were detected by reverse transcription real-time fluorescence quantitative polymerase chain reaction.The expression levels of CaMKⅡ,NF-κB p65,IL-1β,IL-6,TNF-α,and IL-10 in the hippocampal CA1 region were detected by immunofluorescence.Relative expression levels of CaMKⅡ and NF-κB p65 protein in the hippocampus were detected by Western blotting assay. Results:Compared with the blank control group,CUMS rats showed decreased numbers of squares crossed and upright standings and consumption rate of sucrose(P<0.01),and increased swimming immobility time(P<0.01);obvious inflammatory injury in hippocampal CA1 region;increased levels of IL-1β,IL-6,and TNF-α in the hippocampus and serum(P<0.01),and decreased levels of IL-10 and 5-HT(P<0.01);decreased relative expression levels of miR-152-3p and miR-145-5p in the hippocampus(P<0.01)and increased relative mRNA and protein expression levels of CaMKⅡ and NF-κB p65 in the hippocampus(P<0.01),increased mean fluorescence intensities of CaMKⅡ,NF-κB p65,IL-1β,IL-6,and TNF-α(P<0.01),and decreased mean fluorescence intensity of IL-10(P<0.01)in hippocampal CA1 area.Compared with the model group,rats in the acupuncture group and the fluoxetine group had increased numbers of squares crossed and upright standings and consumption rate of sucrose(P<0.01),and significantly reduced swimming immobility time(P<0.01);reduced neuroinflammatory reaction in hippocampal CA1 region;decreased levels of IL-1β,IL-6,and TNF-α(P<0.01),and increased levels of IL-10 and 5-HT(P<0.01)in the hippocampus and serum;increased relative expression levels of miR-152-3p and miR-145-5p in the hippocampus(P<0.01),and decreased relative mRNA and protein expression levels of CaMKⅡ and NF-κB p65 in the hippocampus(P<0.01);decreased mean fluorescence intensities of CaMKⅡ,NF-κB p65,IL-1β,IL-6,and TNF-α in hippocampal CA1 area(P<0.01),while increased mean fluorescence intensity of IL-10(P<0.01).Relative expression levels of miR-152-3p and miR-145-5p in rat hippocampus were negatively correlated with CaMKⅡ mRNA(-1<r<0,P<0.001),while CaMKⅡ mRNA was positively correlated with NF-κB p65 mRNA(0<r<1,P<0.001). Conclusion:The mechanism of the governor vessel-unblocking and mind-regulating acupuncture method in treating depression may be related to the upregulation of miR-152-3p and miR-145-5p expression levels in the hippocampus and the inhibition of neuritis responses caused by the CaMKⅡ/NF-κB signaling pathway in the CA1 region.关键词
针刺疗法/通督调神/微小RNAs/白细胞介素/肿瘤坏死因子-α/神经炎症/抑郁/大鼠Key words
Acupuncture Therapy/Governor Vessel-unblocking and Mind-regulating/MicroRNAs/Interleukins/Tumor Necrosis Factor-α/Neuroinflammation/Depression/Rats分类
医药卫生引用本文复制引用
赵艳标,宋小鸽,孙培养,邹玲,钱婷婷,储易凡,蒋涛,杨燕萍..针刺对抑郁症模型大鼠海马miR-152-3p、miR-145-5p及CaMKⅡ/NF-κB信号通路的影响[J].针灸推拿医学（英文版）,2026,24(3):213-228,16.基金项目
This work was supported by the Key Project of Natural Science Research in Colleges and Universities of Anhui Province(安徽省高等学校自然科学研究重点项目,No.2023AH050740) （安徽省高等学校自然科学研究重点项目,No.2023AH050740）
Anhui Province Clinical Medical Research and Transformation Special Project(安徽省临床医学研究转化专项,No.202304295107020096) （安徽省临床医学研究转化专项,No.202304295107020096）
JIANG Tao's Studio of Famous Traditional Chinese Medicine Practitioner from Anhui Province[安徽省名中医蒋涛工作室,No.中发展(2020)10 号] （2020）
National Project for Building Superior Specialized Clinics in Traditional Chinese Medicine[国家中医优势专科建设 项目,No.财社(2023)29号]. （2023）
