肿瘤防治研究2026，Vol.53Issue(6)：475-481,7.DOI:10.3971/j.issn.1000-8578.2026.25.0971
胃癌患者肠道菌群失调与细胞因子表达异常的关联分析及临床意义
Correlation and Clinical Significance of Intestinal Microbiota Dysbiosis and Abnormal Cytokine Expression in Patients with Gastric Cancer
摘要
Abstract
Objective To explore the correlation between alterations in intestinal microbiota composition and abnormal cytokine expression in patients with gastric cancer.Methods Fecal and peripheral blood samples were collected from 81 patients with gastric cancer(gastric cancer group)and 85 healthy subjects(healthy control group).Fecal bacterial DNA was sequenced through 16S rRNA gene amplicon sequencing,and the serum levels of 17 cytokines were measured with the AimPlex multiplex assay technology:IL-1β,IL-2,IL-4,IL-6,IL-8,IL-10,IL-12p70,IL-15,IL-17A,TNF-α,TNF-β,GM-CSF,G-CSF,IFN-γ,IP-10,MCP-1,and VEGF-A.Results In the gastric cancer group,the abundances of 11 butyrate-producing bacteria,including certain genera of the Lachnospiraceae family(such as Lachnospira and Roseburia)and Faecalibacterium,considerably decreased.By contrast,the abundances of nine bacterial taxa,including Escherichia-Shigella,Streptococcus,Veillonella,and Parabacteroides,significantly increased.Correlation analysis showed that the decrease in the abundance of butyrate-producing bacteria were significantly negatively correlated with the high expression levels of pro-angiogenic and immunosuppressive cytokines in the gastric cancer group,including IP-10,IL-10,MCP-1,IL-8,IL-6,and VEGF-A.However,the decrease was significantly positively correlated with the downregulation of antitumor immune-related cytokines,including IL-2,IL-17A,IL-1β,TNF-β,and GM-CSF.Conversely,the enriched bacterial taxa exhibited opposite correlation patterns with these cytokines.Conclusion A close correlation is observed between intestinal microbiota dysbiosis and abnormal cytokine expression in patients with gastric cancer.The findings suggest that restoring the intestinal microbiota through fecal microbiota transplantation is a promising strategy for regulating cytokines and is a potential research direction for the adjuvant therapy of gastric cancer.关键词
胃癌/肠道菌群/细胞因子/产丁酸菌Key words
Gastric cancer/Intestinal microbiota/Cytokine/Butyrate-producing bacteria分类
医药卫生引用本文复制引用
戚宇锋,苏文,蒋华..胃癌患者肠道菌群失调与细胞因子表达异常的关联分析及临床意义[J].肿瘤防治研究,2026,53(6):475-481,7.基金项目
Key Projects of Changzhou Medical Center of Nanjing Medical University(No.CMCM202402) （No.CMCM202402）
The Special Foundation for Talents of Shanxi Province(No.201705D211021) 南京医科大学常州医学中心重点项目(CMCM202402) （No.201705D211021）
山西省科技厅优秀人才科技创新项目(201705D211021) （201705D211021）
