中南民族大学学报（自然科学版）2026，Vol.45Issue(5)：605-612,8.DOI:10.20056/j.cnki.ZNMDZK.20260732
Nafion-AuNPs修饰碳纤维微电极构建及漆黄素检测应用研究
Construction of Nafion-AuNPs modified carbon fiber microelectrode and application in detection of fisetin
摘要
Abstract
Nafion-AuNPs/CFME composite electrochemical sensor was constructed by using Nafion and gold nanoparticles(AuNPs)as modified materials for carbon fiber electrode(CFME),which established a rapid and simple electrochemical analysis method for the detection of fisetin.The morphology of the modified electrode was characterized by scanning electron microscopy(SEM),and the detection conditions of fisetin were optimized.The electrochemical properties and electrochemical reaction process of fisetin at the modified electrode were investigated by electrochemical impedance spectroscopy(EIS),differential pulse method(DPV)and potentiostatic deposition method(CV).The results showed that under the optimal experimental conditions(pH 3.0,sweeping speed v=100 mV/s),the linear response range of fisetin were 0.01～0.10 μmol/L and 0.10～10 μmol/L,the detection limit(LOD,S/N=3)was 0.006 μmol/L,and the quantification limit(LOQ,S/N=10)was 0.020 μmol/L.The content of fisetin in the leaves of Cotinus coggygria var.pubescens Engl.was measured to be 1.173%using the modified electrode,with a spiked recovery rate ranging from 97.61%to 99.22%(RSD<5.0%).The construction of the modified electrode provides a rapid,stable and accurate detection method for the quantitative detection of fisetin,which is expected to be widely applied in the component analysis of small molecule compounds of Chinese herbal medicine.关键词
Nafion材料/纳米金/碳纤维微电极/漆黄素Key words
Nafion/gold nanoparticles/carbon fiber microelectrode/fisetin分类
化学化工引用本文复制引用
李钰,方涛,黄先菊,Uchkun Ishimov,Ghulam Murtaza,程寒..Nafion-AuNPs修饰碳纤维微电极构建及漆黄素检测应用研究[J].中南民族大学学报（自然科学版）,2026,45(5):605-612,8.基金项目
湖北省国际科技合作项目(2024EHA054) （2024EHA054）
湖北省国际科技合作基地(SH2311) （SH2311）
中南民族大学学术创新团队经费项目资助(XTZ24025) （XTZ24025）
国家重点研发计划(2024YFC3506704) （2024YFC3506704）
