中南民族大学学报（自然科学版）2026，Vol.45Issue(5)：622-629,8.DOI:10.20056/j.cnki.ZNMDZK.20260730
重组表达S100A9蛋白枯草芽孢杆菌对哮喘小鼠的干预作用
The inhibitory effects of a recombinant Bacillus subtilis expressing mouse S100A9 protein on asthmatic mice
摘要
Abstract
To investigate the therapeutic effect of S100A9 recombinant Bacillus subtilis on asthma,the effects and optimal dosage of S100A9 recombinant Bacillus subtilis was explored using the OVA-induced asthmatic mouse from the molecular expression and pathological levels.Compared with the groups of asthma and vector control,the low-dose recombinant bacteria group(1.02×107 CFU/mL)showed a significant reduction in actions of wheezing and arching back,an increase in body weight,and a reduction in lung coefficients;compared with the low-dose,the effects were weaker in the medium-dose-treated group(1.18×108 CFU/mL)and the high-dose-treated group(1.25×109 CFU/mL);pathological sections showed a significant reduction of inflammatory cells infiltration in the airways,and a reduction of thickness of the bronchial tubes(P<0.05);and the expressions of IL-5,IL-6,and IL-13 were significantly decreased in the lung tissues;the mRNA levels of Muc5ac and Muc5b were also significantly reduced(P<0.05);the levels of IL-6 and TNF-α in the serum of mice in the low-dose recombinant bacteria-treated group was significantly reduced;the content of SOD and CAT enzymes in the lung tissues were increased,and there was a significant recovery of the MDA indexes;the number of eosinophils in the alveolar lavage fluid was significantly reduced(P<0.05).The above results indicated that low-dose S100A9 recombinant Bacillus subtilis had a better treating effects on asthma.关键词
S100A9/哮喘/枯草芽孢杆菌/气道炎症Key words
S100A9/asthma/Bacillus subtilis/inflammation of the airways分类
生物科学引用本文复制引用
魏轩,李婷,李甜甜,丁明玲,彭勇波..重组表达S100A9蛋白枯草芽孢杆菌对哮喘小鼠的干预作用[J].中南民族大学学报（自然科学版）,2026,45(5):622-629,8.基金项目
国家自然科学基金资助项目(31371307) （31371307）
中南民族大学研究生教育教学研究项目(YJS22117) （YJS22117）
动物胚胎工程及分子育种湖北省重点实验室开放课题资助项目(KLAEMB-2024-01) （KLAEMB-2024-01）
