中南民族大学学报（自然科学版）2026，Vol.45Issue(5)：630-639,10.DOI:10.20056/j.cnki.ZNMDZK.20260733
复方赤石脂禹余粮散对小鼠止泻作用机制研究
Mechanism of anti-diarrheal effect of compound Halloysitum Rubrum and Limonitum Powder in mice
摘要
Abstract
The study explores the mechanism of anti-diarrheal effect of Compound Halloysitum Rubrum and Limonitum Powder in mice.The main element content was detected by ICP-OES,and the hygroscopicity was determined by the hygroscopic rate method.A total of 70 ICR mice were randomly divided into normal,model,Halloysitum Rubrum and Limonitum Decoction,Halloysitum Rubrum Powder,Limonitum Powder,Halloysitum Rubrum and Limonitum Powder,and positive control groups.Diarrhea models were prepared by gavage with castor oil,and the intestinal Evan's blue propulsion rate was measured.The pathological changes in the small and cecal intestinal wall tissues were observed,and the urea nitrogen,zinc ions,tumor necrosis factor-α(TNF-α)content,and Na+-K+ATPase activity were detected.Immunohistochemistry was used to detect the protein expression of interleukin-6(IL-6)and interleukin-1β(IL-1β),and 16S rRNA sequencing was performed on cecal contents.The results showed that the main elements of Halloysitum Rubrum and Limonitum Powder are Fe and Al,followed by Ca,Mn,Mg,Zn,etc.It significantly inhibited the intestinal Evan's blue propulsion rate in diarrheal mice,reduced intestinal wall cell damage and serum TNF-α content,increased intestinal wall cell Na+-K+ATPase activity and blood zinc ion concentration,and decreased the protein expression of IL-6 and IL-1β in intestinal wall tissues.It also reduced the relative abundance of Staphylococcus and Helicobacter while increasing the abundance of beneficial bacteria such as Lactobacillus,Faecalibacterium,and Bifidobacterium.Therefore,Halloysitum Rubrum and Limonitum Powder exerts its anti-diarrheal effect by supplementing trace elements,adsorbing inflammatory exudates,and regulating the balance of intestinal flora.关键词
赤石脂禹余粮散/止泻/肠道菌群/炎症/元素含量Key words
Compound Halloysitum Rubrum and Limonitum Powder/anti-diarrheal/intestinal microbiota/inflammation/element content分类
医药卫生引用本文复制引用
李娟,王晓飞..复方赤石脂禹余粮散对小鼠止泻作用机制研究[J].中南民族大学学报（自然科学版）,2026,45(5):630-639,10.基金项目
湖北省自然科学基金资助项目(2024AFD224,2024AFD273) （2024AFD224,2024AFD273）
