植物遗传资源学报2026，Vol.27Issue(6)：1092-1102,11.DOI:10.13430/j.cnki.jpgr.20260226002
玉米抗茎腐病主效新位点qRfg3.03的遗传定位
The Genetic Mapping of A Novel Major Locus,qRfg3.03,Conferring Resistance to Stalk Rot in Maize
摘要
Abstract
Maize stalk rot is a destructive disease prevalent across major corn production regions in China,posing a significant threat to both yield and grain quality.Therefore,the identification of elite germplasm and underlying gene resources for stalk rot resistance are of great significance.In this study,an elite maize inbred line CNH3323,previously screened for high resistance to maize stalk rot via large-scale germplasm accurate phenotyping,was used as the resistant parent to construct two segregating populations,including 280 F2∶3 families and 410 doubled haploid(DH)homozygous lines,respectively.The phenotypic evaluations for stalk rot resistance were conducted for two segregating populations under both natural infection and artificial inoculation conditions across two locations.Based on the genotyping of segregating populations and the construction of linkage maps,QTL mapping for maize stalk rot resistance was performed.The results showed that a total of 24 QTLs associated with stalk rot resistance were detected across different environments,distributed on eight chromosomes except for chromosomes 5 and 8.Notably,an environment stable QTL with major-effect,designated as qRfg3.03,was consistently mapped to the bin3.03 region on chromosome 3 using different populations,which explained up to 30.36％of the phenotypic variance.This study provides important candidate loci for the genetic dissection of maize stalk rot resistance and also offers valuable gene resources for maize improvement and the breeding for resistance to stalk rot,which holds significant theoretical research and practical application value.关键词
玉米/茎腐病/QTL/qRfg3.03Key words
maize/stalk rot/QTL/qRfg3.03引用本文复制引用
王宇飞,王天宇,杨德光,李永祥,张登峰,刘旭洋,李振菊,何冠华,石云素,黎裕,李春辉,李会勇..玉米抗茎腐病主效新位点qRfg3.03的遗传定位[J].植物遗传资源学报,2026,27(6):1092-1102,11.基金项目
重庆市政府与中国农科院战略合作项目(KYLX20250500035) （KYLX20250500035）
国家重点研发计划(2021YFD1200700) （2021YFD1200700）
现代农业产业技术体系(CARS-02-04) （CARS-02-04）
中国农业科学院科技创新工程(CAAS-CSCB-202403)Strategic Cooperation Project between Chongqing Municipal Government and the Chinese Academy of Agricultural Sciences(KYLX20250500035) （CAAS-CSCB-202403）
National Key R&D Program of China(2021YFD1200700) （2021YFD1200700）
China Agriculture Research System(CARS-02-04) （CARS-02-04）
Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSCB-202403) （CAAS-CSCB-202403）
