植物遗传资源学报2026，Vol.27Issue(6)：1116-1127,12.DOI:10.13430/j.cnki.jpgr.20260110002
水稻木聚糖合酶基因IRX10在镉积累中的作用
The Role of Xylan Synthase-Associated Gene IRX10 in Cadmium Accumulation in Rice
摘要
Abstract
To address the food safety risks posed by cadmium(Cd)contamination in rice,we identified the role of the cell wall-related gene IRX10(Irregular xylem 10)in regulating Cd uptake and allocation in rice,and validated its potential as a promising candidate gene for breeding rice varieties with low-Cd accumulation.We generated gene-edited rice transgenic lines using the CRISPR/Cas9 system and performed Cd treatments to determine Cd content and its distribution patterns in various rice tissues.Furthermore,by integrating gene expression profiling and cell wall composition analysis,the underlying regulatory mechanism was systematically investigated.The results showed that IRX10 exhibited distinct expression patterns in different tissues.The IRX10 promoter region harbored elements associated with metal and stress responses,indicating its role in metal stress responses.When treated with Cd,the irx10 mutants showed a significantly reduced Cd accumulation in roots and shoots compared with the wild type,but phenotypic differences were not pronounced.Under Cd stress conditions,the irx10 mutants exhibited significantly decreased Cd content in brown rice but increased Cd content in hulls compared with the wild type.No significant differences in zinc(Zn)content were observed in either brown rice or hulls between the mutant and the wild type.Furthermore,the expression of Cd-related genes was changed in multiple tissues in the mutant,leading to reduced Cd loading towards the grain and enhanced Cd accumulation in the hull.Moreover,the altered hemicellulose in the irx10 hull exhibited enhanced Cd-fixing capacity.Natural variation analysis identified advantageous haplotypes associated with low Cd accumulation.Therefore,the loss of IRX10 function can reduce Cd accumulation in brown rice without significant alteration in Zn content by retaining more Cd in the hull,which offers a new candidate gene for breeding low-Cd rice varieties.关键词
水稻/镉污染/细胞壁/籽粒镉积累Key words
rice/cadmium pollution/cell wall/cadmium accumulation in grains引用本文复制引用
党正君,杨潇,田赴浩,王美晗,阮楠,王晔,李丰成,陈温福..水稻木聚糖合酶基因IRX10在镉积累中的作用[J].植物遗传资源学报,2026,27(6):1116-1127,12.基金项目
国家重点研发计划(2024YFD1201003-3)National Key Research and Development Program of China(2024YFD1201003-3) （2024YFD1201003-3）
