植物遗传资源学报2026，Vol.27Issue(6)：1266-1279,14.DOI:10.13430/j.cnki.jpgr.20260129002
小麦SPFH家族的鉴定及条锈病胁迫响应特征分析
Identification of the SPFH Family in Wheat and Its Response to Stripe Rust Stress
摘要
Abstract
Wheat is a globally important food crop that supports the food security of approximately 30％of the world's population.This study systematically identified the SPFH family in common wheat(Triticum aestivum L.),comprehensively analyzed its sequence characteristics,protein structure,and response patterns to biotic stress(specifically wheat stripe rust),and revealed its potential biological functions in wheat stress resistance.By comparative genomic data from Arabidopsis thaliana,rice(Oryza sativa L.),and maize(Zea mays),we identified 60 SPFH family members at the whole-genome level in wheat.The results show that SPFH family proteins generally exhibit acidic and hydrophilic characteristics.Gene structure analysis revealed that intron lengths and numbers are similar within HIR and Flot subfamilies,whereas significant heterogeneity in sequence length exists among subfamilies.Promoter analysis further indicated that this family is enriched in cis-regulatory elements associated with growth,development,and multiple stress responses.Evolutionary analysis based on phylogeny and structural modeling yielded highly consistent results,revealing structural conservation within subfamilies and a conserved evolutionary pattern across the family.Intraspecific and interspecific synteny analyses suggested that the family underwent chromosomal translocation and gene expansion during polyploidization,with a universal 1∶3 orthologous ratio between monocots and wheat.Nucleotide diversity and heteromeric polymerization analyses uncovered the extreme conservation of the TaHIR,TaSLP,and TaPHB subfamilies,and revealed the functional potential of most members to form stable heteromeric complexes.Transcriptome and qRT-PCR analyses revealed that the SPFH family members exhibited complex expression dynamics under Puccinia striiformis f.sp.tritici stress:although the overall expression was down-regulated at the macroscopic transcriptional level,key members were significantly induced and up-regulated in the susceptible variety Avocet S.Collectively,the SPFH family is highly conserved in evolution and sensitive to biotic stress responses.This study provides important candidate targets for dissecting the molecular mechanism of wheat disease resistance and theoretical support for crop resistance improvement.关键词
小麦/SPFH家族/全基因组鉴定/小麦条锈病/表达分析Key words
wheat/SPFH family/genome-wide identification/wheat stripe rust/expression analysis引用本文复制引用
柯珂,徐舒畅,董海涛,孙唯航,张恒瑞,韩德俊,李春莲,吴建辉..小麦SPFH家族的鉴定及条锈病胁迫响应特征分析[J].植物遗传资源学报,2026,27(6):1266-1279,14.基金项目
国家自然科学基金面上项目(32272088) （32272088）
青海省重点研发与转化计划项目(2025-NK-129) （2025-NK-129）
陕西省自然科学基础研究计划(2019JCW-18,2020JCW-16)National Natural Science Foundation of China General Program(32272088) （2019JCW-18,2020JCW-16）
Qinghai Province Key R&D and Transformation Program Project(2025-NK-129) （2025-NK-129）
Shaanxi Province Natural Science Basic Research Program(2019JCW-18,2020JCW-16) （2019JCW-18,2020JCW-16）
