植物遗传资源学报2026，Vol.27Issue(6)：1292-1304,13.DOI:10.13430/j.cnki.jpgr.20260130001
甘蓝NAC基因家族鉴定及热胁迫下表达分析
Genome Wide Identification and Expression Analysis of the NAC Gene Family in Brassica oleracea under Heat Stress
摘要
Abstract
The NAC transcription factor is one of the largest gene families in plants,playing a crucial role in plant growth,development,and stress response.To elucidate the characteristics of the NAC gene family in cabbage and its regulatory mechanism underlying thermotolerance,this study employed bioinformatics methods to conduct a genome-wide identification of NAC genes in cabbage,and systematically analyzed their physicochemical properties,chromosomal distribution,gene structure,phylogenetic relationships,and promoter cis-acting elements.The expression patterns of cabbage NAC genes in different tissues and under high temperature stress treatment were examined by combining transcriptomic data and quantitative real-time PCR(qRT-PCR).Furthermore,the subcellular localization of the BoNAC36 protein was analyzed using a transient expression system in Nicotiana benthamiana leaves.The results showed that 204 BoNAC genes were identified in the cabbage genome,the number of encoded amino acids ranging from 154 to 855 aa,molecular weights ranging from 17752.17 Da to 95180.96 Da,and isoelectric points between 4.33 and 9.83.The 204 BoNAC genes were unevenly distributed across nine chromosomes,with the highest number of genes located on chromosome 5.Based on the classification method of Arabidopsis NAC protein,the BoNAC genes were divided into 16 subfamilies,with members within the same subfamily exhibiting similarities in conserved motifs and gene structures.The promoter regions of BoNAC genes contained multiple light-responsive elements,hormone-responsive elements,stress-responsive elements,and growth and development regulatory elements.Tissue expression analysis revealed that BoNAC genes with higher expression levels in roots belonged to the TIP and ATAF subfamilies.Quantitative real-time PCR analysis showed that 14 BoNAC genes were consistently and highly expressed at 6 h and 12 h under heat stress,among which BoNAC36 exhibited the most significant upregulated expression.Subcellular localization results indicated that BoNAC36 was localized in the nucleus.This study provides an important data foundation for further in-depth analysis of the function of NAC genes in cabbage under heat stress.关键词
甘蓝/NAC基因家族/生物信息学/表达分析/亚细胞定位Key words
cabbage/NAC gene family/bioinformatics/expression analysis/subcellular localization引用本文复制引用
王敏,陈锦秀,薄天岳,杜子龙,顾大国,朱晓炜..甘蓝NAC基因家族鉴定及热胁迫下表达分析[J].植物遗传资源学报,2026,27(6):1292-1304,13.基金项目
国家重点研发计划(2023YFD1201501) （2023YFD1201501）
国家自然科学基金(32502709) （32502709）
上海市农业科学院卓越团队建设项目[农科卓2022(007)]National Key R&D Program of China(2023YFD1201501) （007）
National Natural Science Foundation of China(32502709) （32502709）
The Shanghai Academy of Agricultural Sciences Excellent Team Project[NongKeZhuo 2022(007)] （007）
