植物营养与肥料学报2026，Vol.32Issue(6)：1216-1225,10.DOI:10.11674/zwyf.2025418
土地利用与施肥对黑土N2O排放的影响:基于15N示踪的硝化与反硝化贡献分析
Effects of land use and fertilization on N2O emissions from black soil:contributions of nitrification and denitrification based on 15N tracing
摘要
Abstract
[Objectives]Exploring the impacts of land use patterns and fertilization on N2O emissions and their generation processes can deepen our understanding of the emission factors and mechanisms of N2O in soil,providing a scientific basis for rational fertilization and greenhouse gas reduction.[Methods]In this study,a 15N pair tracer(15NH4+and 15NO3-)indoor soil incubation experiment was carried out under 60%water holding capacity(WHC)at 25℃.The tested soils were collected from a fallow land(FL)and treatment plots of no fertilizer control(CK),nitrogen and phosphorous fertilization(NP),and combined application of NP and organic fertilizer(NPM)in a localized experiment.During the seven days of incubation,the N2O emission rate and 15N enrichment of N2O were investigated every day.The content and 15N enrichment of NH4+and NO3-in soils were measured at 0,1,3,5,and 7 days of incubation for constructing the FLUAZ model,which was used to fit the gross nitrification and mineralization rate,and to investigate N2O production processes.[Results]Land use patterns and fertilization regimes significantly influenced N2O emissions.The N2O emission rate of fallow land was N2O-N 23.9 ng/(kg∙h),which was significantly lower than that of farmland soil.The N2O emission rate in the NP treatment[N2O-N 146 ng/(kg∙h)]was 1.87 times that of the CK treatment[N2O-N 77.9 ng/(kg∙h)],while the NPM treatment had the highest N2O emission rate[N2O-N 222 ng/(kg∙h)],which was 2.85 and 1.52 times those of the CK and NP treatments,respectively.The order of N2O emission ratios among treatments during the incubation period was NPM>NP>CK>FL,with values ranging from 0.012%to 0.029%.N2O emitted from the soil originated from both nitrification and denitrification processes,but the contribution of nitrification to N2O emissions ranged from 83.1%to 89.4%.A significant positive correlation was observed between the N2O emission rate and the gross nitrification rate,indicating that nitrification was the primary pathway for N2O emissions.The contributions of denitrification to N2O emissions in FL,CK,and NP treatments were 10.6%,10.2%,and 13.1%,respectively,while the contribution significantly increased to 16.9%in the NPM treatment.[Conclusions]Land reclamation and utilization significantly enhanced the nitrification and denitrification processes of nitrogen in the soil,thereby increasing N2O emissions.Fertilization significantly promoted N2O emissions from farmland soil,particularly in the treatment of nitrogen and phosphorus combined with organic fertilizer,where N2O emissions were significantly higher than those in the no-fertilizer and nitrogen and phosphorus fertilizer treatments.Nitrification was the primary pathway for N2O emissions from black soil,while the contribution of denitrification could not be overlooked.Fertilization,especially the combination of nitrogen and phosphorus fertilizers with organic fertilizer,significantly increased the contribution rate of denitrification to N2O emissions.Therefore,controlling nitrification and denitrification processes is crucial for reducing nitrogen fertilizer losses and lowering N2O emissions.关键词
硝化/反硝化/N2O/土地利用方式/施肥Key words
nitrification/denitrification/N2O/land use method/fertilization引用本文复制引用
郎漫,毛涛,张君岳,钟文,李平..土地利用与施肥对黑土N2O排放的影响:基于15N示踪的硝化与反硝化贡献分析[J].植物营养与肥料学报,2026,32(6):1216-1225,10.基金项目
中央土壤污染防治资金项目(新集采单[2021]1468). （新集采单[2021]1468）
