中医临床研究2026，Vol.18Issue(10)：1-9,9.DOI:10.3969/j.issn.1674-7860.2026.10.001
网络药理学和分子对接分析水蛭调控铁死亡治疗痛风的作用机制
An analysis on the mechanism of Shuizhi regulating ferroptosis in the treatment of gout based on network pharmacology and molecular docking
摘要
Abstract
Objective:To analyze the mechanism of Shuizhi(Hirudo)regulating ferroptosis in the treatment of gout by using network pharmacology and molecular docking techniques,and to provide a theoretical basis for clinical application.Methods:Based on network pharmacology and molecular docking techniques,the active components of Shuizhi,as well as the targets related to gout and ferroptosis,were screened through databases such as HERB,GeneCards,OMIM,Drugbank,and FerrDb.The intersection targets of the three were obtained using the Venny platform,the protein interaction network(PPI)was constructed by STRING,and the core genes were screened by Cytoscape.The key pathways were enriched and analyzed through GO and KEGG,and the binding activity between the active ingredients and the core targets was verified in combination with molecular docking.Results:A total of 21 active components and 390 targets of Shuizhi were screened out,3 455 gout targets and 728 ferroptosis genes were obtained,and 27 intersection targets of the three were identified.PPI network analysis identified STAT3,EGFR,PTGS2,AR,IL-1B,PPARG,mTOR,and PPARA as the core genes.The molecular docking of ursolic acid to the core target was stable.KEGG pathway enrichment analysis suggested that the active pathways involved cancer-related pathways,ErbB signaling pathways,and HIF-1 signaling pathways.Conclusion:Shuizhi may treat gout by acting on core genes to regulate ferroptose-related inflammatory pathways and cancer signaling pathways.关键词
水蛭/铁死亡/痛风/网络药理学/分子对接Key words
Shuizhi/Ferroptosis/Gout/Network pharmacology/Molecular docking分类
医药卫生引用本文复制引用
施明华,班正涛,龚德飞,刘汝专..网络药理学和分子对接分析水蛭调控铁死亡治疗痛风的作用机制[J].中医临床研究,2026,18(10):1-9,9.基金项目
广西中医药大学青年项目(2022QN025) （2022QN025）
2024年广西名中医传承工作室建设项目-刘汝专广西名中医工作室(GZY2024018) （GZY2024018）
广西壮族自治区中医药管理局自筹经费科研课题(GXZYZ20240122) （GXZYZ20240122）
广西壮族自治区中医药管理局自筹经费科研课题(GXZYZ20210480). （GXZYZ20210480）
