中药新药与临床药理2026，Vol.37Issue(6)：1036-1042,7.DOI:10.19378/j.issn.1003-9783.2026.06.006
温阳活血方调节FOXA2/LIF/HOXA10通路增加子宫腺肌病在位内膜胞饮突数量的机制
Mechanism of Wenyang Huoxue Formula in Increasing the Number of Pinopodes in the Eutopic Endometrium of Adenomyosis Mice by Regulating the FOXA2/LIF/HOXA10 Pathway
摘要
Abstract
Objective To investigate the effect of Wenyang Huoxue Formula on improving pinopodes in adenomyosis mice and its underlying mechanism.Methods A mouse model of adenomyosis was established using the tamoxifen method.Mice were divided into normal control group,model group,GnRH-α(gonadotropin-releasing hormone agonist)group,and low-,medium-,and high-dose Wenyang Huoxue Formula groups.Each group received the corresponding intervention for 8 weeks.The number of pinopodes was observed by scanning electron microscopy.The expression levels of Forkhead box A2(FOXA2),Leukemia Inhibitory Factor(LIF),and homeobox A10(HOXA10)in the pinopode formation pathway were detected by Western Blot and immunohistochemistry.The mRNA expression levels of FOXA2 and HOXA10 in the mouse endometrium were measured by q-PCR.Results Compared with the normal control group,no obvious pinopodes were observed in the endometrium of adenomyosis model mice(P＜0.05).The protein expression levels of FOXA2,LIF,and HOXA10 in the model group were significantly lower than those in the normal control group(P＜0.05),and the mRNA expression levels of FOXA2 and HOXA10 were also significantly decreased(P＜0.01).Compared with the model group,all doses(low,medium,and high)of Wenyang Huoxue Formula significantly increased the number of pinopodes in the endometrium of adenomyosis mice(P＜0.05).The high-dose Wenyang Huoxue Formula group significantly upregulated the protein expression levels of FOXA2,LIF,and HOXA10(P＜0.05)as well as the mRNA expression levels of FOXA2 and HOXA10(P＜0.01)compared with the model group.Furthermore,the effect of the high-dose Wenyang Huoxue Formula was similar to that of GnRH-α(P＞0.05).Conclusion Wenyang Huoxue Formula can activate the FOXA2/LIF/HOXA10 pathway to increase the number of pinopodes in the endometrium of adenomyosis mice,providing a mechanistic reference for improving endometrial receptivity in patients with adenomyosis-associated infertility.关键词
温阳活血方/FOXA2/LIF/HOXA10 通路/胞饮突/不孕/子宫内膜容受性/子宫腺肌病/小鼠Key words
Wenyang Huoxue Formula/FOXA2/LIF/HOXA10 pathway/pinopodes/endometrial receptivity/adenomyosis/mice分类
医药卫生引用本文复制引用
范伟森,马昊天,赵瑞华,杨艳环,李娟梅..温阳活血方调节FOXA2/LIF/HOXA10通路增加子宫腺肌病在位内膜胞饮突数量的机制[J].中药新药与临床药理,2026,37(6):1036-1042,7.基金项目
北京市自然科学基金项目(7252260) （7252260）
中国中医科学院科技创新工程重点协同攻关项目(CI2022C003). （CI2022C003）
