中药药理与临床2026，Vol.42Issue(5)：25-32,8.
鸢芩胶囊对脂多糖诱导小鼠急性肺损伤的作用及肠道菌群的影响
Protective Effects of Yuanqin Capsules on Lipopolysaccharide-Induced Acute Lung Injury in Mice and Their Impact on Gut Microbiota
摘要
Abstract
Objective:To explore the mechanism of action of Yuanqin(鸢芩)Capsules on lipopolysaccharide(LPS)-induced acute lung injury(ALI)in mice and its effect on the gut microbiota.Methods:Thirty-six male C57BL/6J mice were randomly divided into normal control group,model control group,prednisone acetate 50 mg/kg group,and Yuanqin Capsules 1.95,3.90,and 7.80 g/kg groups.The ALImodel was established by intratracheal instillation of LPS.The total cell count and white blood cell count in bronchoalveolar lavage fluid(BALF)were measured.Hematoxylin and eosin(HE)staining was used to observe the histopathological changes in lung tissue.Enzyme-linked immunosorbent as-say(ELISA)was used to detect the levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in lung tissue.Western blot was used to detect the protein expression levels of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT)and their phosphorylated forms(p-PI3K,p-AKT),IL-6,TNF-α,inducible nitric oxide synthase(iNOS),man-nose receptor type C-1(MRC1)and arginase-1(Arg1)in lung tissue.16S rRNA gene sequencing was used to detect the composition and diversity of the gut microbiota in fecal samples.Results:Compared with the normal control group,the model control group exhibited significantly increased inflammatory cell count in BALF,lung injury score,and IL-6 and TNF-α levels(P<0.01).The ratios of p-PI3K/PI3K and p-AKT/AKT,as well as the expressions of Arg1 and MRC1,significantly decreased(P<0.05 or P<0.01),while the protein expressions of iNOS,IL-1β and TNF-α were significantly upregulated(P<0.05 or P<0.01).Compared with the model control group,the total cell count and the white blood cell count in BALF significantly decreased in the Yuanqin Capsules 1.95 g/kg group(P<0.01).The lung injury scores sig-nificantly decreased in the Yuanqin Capsules 1.95 g/kg and 7.80 g/kg groups(P<0.01).The IL-6 levels in lung tis-sue were reduced in all dosage groups(P<0.05 or P<0.01).TNF-α levels significantly decreased in the Yuanqin Cap-sules 3.90 g/kg and 7.80 g/kg groups(P<0.01).In the Yuanqin Capsules 7.80 g/kg group,the protein expression levels of Arg1,MRC1,and the ratios of p-AKT/AKT and p-PI3K/PI3K were upregulated(P<0.05 or P<0.01),while the protein expression levels of TNF-α,iNOS and IL-1β were downregulated(P<0.05 or P<0.01).Gut microbiota a-nalysis showed that the Yuanqin Capsules 7.80 g/kg group significantly remodeled the gut microbiota structure in ALI mice,reduced microbiota diversity,decreased pro-inflammatory genera such as Desulfovibrio and Mucispirillum,and en-riched beneficial bacteria such as Akkermansia.It was also suggested that microbial functions related to inflammation and metabolic pathways might be modulated.Conclusion:Yuanqin Capsules may exert a protective effect against ALI by ac-tivating the PI3K/AKT signaling pathway,downregulating the expression of pro-inflammatory factors,upregulating the an-ti-inflammatory proteins Arg1 and MRC1 in lung tissue,and modulating the structure of the gut microbiota.关键词
鸢芩胶囊/急性肺损伤/脂多糖/磷脂酰肌醇3-激酶/蛋白激酶B通路/肠道菌群Key words
Yuanqin Capsules/Acute lung injury/Lipopolysaccharide/Phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway/Gut microbiota引用本文复制引用
张红,张磊,周静,孟慧心,牛凯琳,杨沛勋,罗亚,杨薇,袁明铭,张雪妮,张秋悦..鸢芩胶囊对脂多糖诱导小鼠急性肺损伤的作用及肠道菌群的影响[J].中药药理与临床,2026,42(5):25-32,8.基金项目
四川省岐黄学者能力提升项目(编号:QHXZ-2023ZL-001) （编号:QHXZ-2023ZL-001）
四川省中医药管理局科学技术研究专项(编号:2024ZD01) （编号:2024ZD01）
四川省中医药科学院事业发展推进项目(编号:SYF-2023-3). （编号:SYF-2023-3）
